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Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution

The objective of this study was to characterize the morphometry and ultrastructure of porcine preantral and antral follicles, especially the lipid component evolution. Ovarian tissue was processed for light microscopy. Ovarian tissue and dissected antral follicles (< 2, 2–4, and 4–6 mm) were also...

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Main Authors: Silva, Renata C., Báo, Sônia Nair, Rôlo, José Luiz Jivago de Paula, Lucci, Carolina Madeira
Format: Artigo
Language: Inglês
Published: Elsevier 2015
Subjects:
Lipídios
Folículos pré-antrais
Suíno
Online Access: http://repositorio.unb.br/handle/10482/17621
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spelling ir-10482-176212020-09-18T18:37:16Z Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution Silva, Renata C. Báo, Sônia Nair Rôlo, José Luiz Jivago de Paula Lucci, Carolina Madeira Lipídios Folículos pré-antrais Suíno The objective of this study was to characterize the morphometry and ultrastructure of porcine preantral and antral follicles, especially the lipid component evolution. Ovarian tissue was processed for light microscopy. Ovarian tissue and dissected antral follicles (< 2, 2–4, and 4–6 mm) were also processed for transmission electron microscopy using routine methods and using an osmium-imidazole method for lipid detection. Primordial follicles (34 ± 5 μm in diameter, mean ± SD) had one layer of flattened-cuboidal granulosa cells around the oocyte, primary follicles (40 ± 7 μm) had a single layer of cuboidal granulosa cells around the oocyte, and secondary follicles (102 ± 58 μm) had two or more layers of cuboidal granulosa cells around the oocyte. Preantral follicle oocytes had many round mitochondria and both rough and smooth endoplasmic reticulum. In oocytes of primordial and primary follicles, lipid droplets were abundant and were mostly located at the cell poles. In secondary and antral follicles, the zona pellucida completely surrounded the oocyte, whereas some microvilli and granulosa cells projected through it. Numerous electron-lucent vesicles and vacuoles were present in the oolemma of secondary and antral follicles. Based on osmium-imidazole staining, most of these structures were shown to be lipid droplets. As the follicle developed, the appearance of the lipid droplets changed from small and black to large and gray, dark or dark with light streaks, suggesting that their nature may change over time. In summary, although porcine follicles and oocytes had many similarities to those of other mammalian species, they were rich in lipids, with lipid droplets with varying morphological patterns as the follicle developed. 2015-02-11T12:22:43Z 2015-02-11T12:22:43Z 2011 Artigo SILVA, Renata C., et al. Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development: lipid component evolution. Theriogenology, v. 76, p. 1647-1657, 2011. Disponível em:<http://www.sciencedirect.com/science/article/pii/S0093691X11003141>. Acesso em: 03 fev. 2015. http://repositorio.unb.br/handle/10482/17621 Inglês Acesso Aberto Este Manuscrito do Autor Aceito para Publicação (AAM) é protegido por direitos autorais e publicado pela Elsevier. Ele esta disponível neste Repositório, por acordo entre a Elsevier e a Universidade de Brasília. As alterações decorrentes do processo de publicação - como a edição, correção, formatação estrutural, e outros mecanismos de controle de qualidade - não estão refletidas nesta versão do texto. A versão definitiva do texto foi posteriormente publicado em [Theriogenology, Volume 76, Número 9, Dezembro 2011, Pages 1647–1657, doi:10.1016/j.theriogenology.2011.06.029]. Você pode baixar, copiar e utilizar de outra forma o AAM para fins não comerciais , desde que sua licença seja limitada pelas seguintes restrições: (1) Você pode usar este AAM para fins não comerciais apenas sob os termos da licença CC- BY- NC-ND. (2) A integridade do trabalho e identificação do autor, detentor dos direitos autorais e editor deve ser preservado em qualquer cópia. (3) Tem de atribuir este AAM no seguinte formato: [acordo na linguagem atribuída, incluindo o link para CC BY-NC-ND licença Digital + DOI do artigo publicado na revista Elsevier ScienceDirect ® da plataforma]. application/pdf Elsevier
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language Inglês
topic Lipídios
Folículos pré-antrais
Suíno
spellingShingle Lipídios
Folículos pré-antrais
Suíno
Silva, Renata C.
Báo, Sônia Nair
Rôlo, José Luiz Jivago de Paula
Lucci, Carolina Madeira
Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
description The objective of this study was to characterize the morphometry and ultrastructure of porcine preantral and antral follicles, especially the lipid component evolution. Ovarian tissue was processed for light microscopy. Ovarian tissue and dissected antral follicles (< 2, 2–4, and 4–6 mm) were also processed for transmission electron microscopy using routine methods and using an osmium-imidazole method for lipid detection. Primordial follicles (34 ± 5 μm in diameter, mean ± SD) had one layer of flattened-cuboidal granulosa cells around the oocyte, primary follicles (40 ± 7 μm) had a single layer of cuboidal granulosa cells around the oocyte, and secondary follicles (102 ± 58 μm) had two or more layers of cuboidal granulosa cells around the oocyte. Preantral follicle oocytes had many round mitochondria and both rough and smooth endoplasmic reticulum. In oocytes of primordial and primary follicles, lipid droplets were abundant and were mostly located at the cell poles. In secondary and antral follicles, the zona pellucida completely surrounded the oocyte, whereas some microvilli and granulosa cells projected through it. Numerous electron-lucent vesicles and vacuoles were present in the oolemma of secondary and antral follicles. Based on osmium-imidazole staining, most of these structures were shown to be lipid droplets. As the follicle developed, the appearance of the lipid droplets changed from small and black to large and gray, dark or dark with light streaks, suggesting that their nature may change over time. In summary, although porcine follicles and oocytes had many similarities to those of other mammalian species, they were rich in lipids, with lipid droplets with varying morphological patterns as the follicle developed.
format Artigo
author Silva, Renata C.
Báo, Sônia Nair
Rôlo, José Luiz Jivago de Paula
Lucci, Carolina Madeira
author_sort Silva, Renata C.
title Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
title_short Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
title_full Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
title_fullStr Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
title_full_unstemmed Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
title_sort ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
publisher Elsevier
publishDate 2015
url http://repositorio.unb.br/handle/10482/17621
_version_ 1680624711604633600
score 13.657419

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