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A simple method for human peripheral blood monocyte Isolation

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient...

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Main Authors: Almeida, Marcos C de, Silva, Alan C, Barral, Aldina, Barral Netto, Manoel
Format: Artigo
Language: English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Subjects:
monocytes
percoll
gradient-isolation
Online Access: http://repositorio.unb.br/handle/10482/25587
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spelling ir-10482-255872019-03-28T14:03:51Z A simple method for human peripheral blood monocyte Isolation Almeida, Marcos C de Silva, Alan C Barral, Aldina Barral Netto, Manoel monocytes percoll gradient-isolation We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes. 2017-12-07T04:32:47Z 2017-12-07T04:32:47Z 2000 Artigo Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000 0074-0276 http://repositorio.unb.br/handle/10482/25587 10.1590/S0074-02762000000200014 en Acesso Aberto application/pdf Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200014&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02762000000200014&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0074-02762000000200014&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic monocytes
percoll
gradient-isolation
spellingShingle monocytes
percoll
gradient-isolation
Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
A simple method for human peripheral blood monocyte Isolation
description We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
format Artigo
author Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
author_sort Almeida, Marcos C de
title A simple method for human peripheral blood monocyte Isolation
title_short A simple method for human peripheral blood monocyte Isolation
title_full A simple method for human peripheral blood monocyte Isolation
title_fullStr A simple method for human peripheral blood monocyte Isolation
title_full_unstemmed A simple method for human peripheral blood monocyte Isolation
title_sort simple method for human peripheral blood monocyte isolation
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2017
url http://repositorio.unb.br/handle/10482/25587
_version_ 1641988447124062208
score 13.657419

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