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Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis

In the animal model of leishmaniasis caused by Leishmania (Leishmania) amazonensis there is a complex mechanism of the host-parasite interaction. The present study was performed to interfere with the inflammatory reaction to the parasites, through immune modulation. Female C5BL/6 isogenic mice were...

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Main Authors: Oliveira, Thiago de Sá, Capp Neto, Mário, Martins, Bernardo José Alves, Rodrigues, Helbert Abe, Antonino, Rosa Maria Parreiras, Magalhães, Albino Verçosa de
Format: Artigo
Language: English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Subjects:
Leishmaniose cutânea
Leishmaniose
Fator de necrose de tumor
Online Access: http://repositorio.unb.br/handle/10482/25588
https://dx.doi.org/10.1590/S0074-02762000000400006
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spelling ir-10482-255882021-04-30T19:34:22Z Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis Oliveira, Thiago de Sá Capp Neto, Mário Martins, Bernardo José Alves Rodrigues, Helbert Abe Antonino, Rosa Maria Parreiras Magalhães, Albino Verçosa de Leishmaniose cutânea Leishmaniose Fator de necrose de tumor In the animal model of leishmaniasis caused by Leishmania (Leishmania) amazonensis there is a complex mechanism of the host-parasite interaction. The present study was performed to interfere with the inflammatory reaction to the parasites, through immune modulation. Female C5BL/6 isogenic mice were used, some of which were inoculated on the right ear and others on the right footpad with 3.10(6) stationary phase promastigotes of the MHOM/BR/PH8 strain of L. (L.) amazonensis, and were allocated in three groups: the first received pentoxifylline 8mg/kg every 12 h, since the first day; the second one received the same dose since the 40th day of infection and a control group that did not receive any treatment. All the ears excised were analyzed to determine the variation in weight between both ears and for histopathological analyses. A quantification of the parasites was done using the limiting dilution assay. A significant reduction of the number of parasites, was observed among the animals treated which had an accordingly significant reduction on the weight of the ears. Pentoxifylline reduced the macrophages propensity to vacuolation and induced a more effective destruction of the parasites by these cells. Moreover, the group that began the treatment later did not show the same effectiveness. 2017-12-07T04:32:48Z 2017-12-07T04:32:48Z 2000-08 Artigo OLIVEIRA, Thiago de Sá et al. Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 4, p. 477-482, jul./ago. 2000. DOI: https://doi.org/10.1590/S0074-02762000000400006. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000400006&lng=en&nrm=iso. Acesso: 19 mar. 2021. http://repositorio.unb.br/handle/10482/25588 https://dx.doi.org/10.1590/S0074-02762000000400006 en Acesso Aberto Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000400006&lng=en&tlng=en. Acesso em: 19 mar. 2021. application/pdf Instituto Oswaldo Cruz, Ministério da Saúde
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Leishmaniose cutânea
Leishmaniose
Fator de necrose de tumor
spellingShingle Leishmaniose cutânea
Leishmaniose
Fator de necrose de tumor
Oliveira, Thiago de Sá
Capp Neto, Mário
Martins, Bernardo José Alves
Rodrigues, Helbert Abe
Antonino, Rosa Maria Parreiras
Magalhães, Albino Verçosa de
Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
description In the animal model of leishmaniasis caused by Leishmania (Leishmania) amazonensis there is a complex mechanism of the host-parasite interaction. The present study was performed to interfere with the inflammatory reaction to the parasites, through immune modulation. Female C5BL/6 isogenic mice were used, some of which were inoculated on the right ear and others on the right footpad with 3.10(6) stationary phase promastigotes of the MHOM/BR/PH8 strain of L. (L.) amazonensis, and were allocated in three groups: the first received pentoxifylline 8mg/kg every 12 h, since the first day; the second one received the same dose since the 40th day of infection and a control group that did not receive any treatment. All the ears excised were analyzed to determine the variation in weight between both ears and for histopathological analyses. A quantification of the parasites was done using the limiting dilution assay. A significant reduction of the number of parasites, was observed among the animals treated which had an accordingly significant reduction on the weight of the ears. Pentoxifylline reduced the macrophages propensity to vacuolation and induced a more effective destruction of the parasites by these cells. Moreover, the group that began the treatment later did not show the same effectiveness.
format Artigo
author Oliveira, Thiago de Sá
Capp Neto, Mário
Martins, Bernardo José Alves
Rodrigues, Helbert Abe
Antonino, Rosa Maria Parreiras
Magalhães, Albino Verçosa de
author_sort Oliveira, Thiago de Sá
title Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
title_short Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
title_full Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
title_fullStr Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
title_full_unstemmed Action of pentoxifylline on experimental cutaneous leishmaniasis due to Leishmania (Leishmania) amazonensis
title_sort action of pentoxifylline on experimental cutaneous leishmaniasis due to leishmania (leishmania) amazonensis
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2017
url http://repositorio.unb.br/handle/10482/25588
https://dx.doi.org/10.1590/S0074-02762000000400006
_version_ 1701480381864214528
score 13.657419

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