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Variability in micronucleus induction with different mutagens applied to several species of fish

Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction t...

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Main Authors: Grisolia, Cesar Koppe, Cordeiro, Célia Maria Torres
Format: Artigo
Language: English
Published: Sociedade Brasileira de Genética 2017
Online Access: http://repositorio.unb.br/handle/10482/25662
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spelling ir-10482-256622019-03-28T14:04:03Z Variability in micronucleus induction with different mutagens applied to several species of fish Grisolia, Cesar Koppe Cordeiro, Célia Maria Torres Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, Tilapia rendalli, Oreochromis niloticus and Cyprinus carpio, were exposed to the clastogens bleomycin (BLM), cyclophosphamide (CP), 5-fluorouracil (5-FU), and mitomycin C (MMC). The binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin B was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes. Micronucleus frequencies induced by CP were significantly greater than their respective controls for the three fish species throughout all treatment periods. During the whole evaluation period (30 days) CP was also the most effective clastogen. In general, until the 14th day of evaluation period T. rendalii was the most sensitive species to clastogens. No difference in micronucleus frequencies among species was observed in the 4th evaluation (at the 30th day). A micronucleus peak was observed at the 7th day after treatment. After the 14th day the frequencies were stabilized. The cytochalasin B experiment was carried out to demonstrate that micronuclei induced in the young kidney erythrocyte cells were detected in the circulating blood 2-4 days later. Este estudo fez uma avaliação da indução de micronúcleos em eritrócitos de sangue periférico de peixes Tilapia rendalli, Oreochromis niloticus e Cyprinus carpio após o tratamento com mitomicina C, ciclofosfamida, 5-fluorouracil e bleomicina. Foram colhidas amostras periódicas de sangue com 2, 7, 14 e 30 dias após o tratamento único. Os tratamentos com citocalasina B tiveram como objetivo analisar as proporções entre células binucleadas/mononucleadas nos diferentes períodos de tratamento, para a comparação com os picos de micronúcleos entre as diferentes espécies, uma vez que o orgão hematopoiético nos peixes é o rim cefálico e o sangue analisado é de origem periférica, o que nos dá uma noção do tempo do ciclo celular dos eritrócitos. A análise das taxas de aumento das freqüências de micronúcleos ao longo do tempo demonstrou que do 2° ao 7°dias elas foram crescentes, decresceram ao 14° dia e mantiveram-se estáveis até ao 30°. Os 4 compostos químicos aumentaram significativamente as freqüências de micronúcleos ao longo de todo o período de tratamento, sendo que a ciclofosfamida e a mitomicina C foram as mais efetivas. A T. rendalli foi a espécie mais sensível a todos os tratamentos, enquanto que C. carpio foi a mais resistente. Entre 2 e 7 dias pós-tratamento observaram-se as maiores induções de micronúcleos. A partir do 14° dia de tratamento as freqüências de micronúcleos tenderam a diminuir e as diferenças entre as espécies não foram significativas. 2017-12-07T04:33:04Z 2017-12-07T04:33:04Z 2000 Artigo Genet. Mol. Biol.,v.23,n.1,p.235-239,2000 1415-4757 http://repositorio.unb.br/handle/10482/25662 10.1590/S1415-47572000000100041 en Acesso Aberto application/pdf Sociedade Brasileira de Genética http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100041&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572000000100041&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1415-47572000000100041&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
description Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, Tilapia rendalli, Oreochromis niloticus and Cyprinus carpio, were exposed to the clastogens bleomycin (BLM), cyclophosphamide (CP), 5-fluorouracil (5-FU), and mitomycin C (MMC). The binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin B was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes. Micronucleus frequencies induced by CP were significantly greater than their respective controls for the three fish species throughout all treatment periods. During the whole evaluation period (30 days) CP was also the most effective clastogen. In general, until the 14th day of evaluation period T. rendalii was the most sensitive species to clastogens. No difference in micronucleus frequencies among species was observed in the 4th evaluation (at the 30th day). A micronucleus peak was observed at the 7th day after treatment. After the 14th day the frequencies were stabilized. The cytochalasin B experiment was carried out to demonstrate that micronuclei induced in the young kidney erythrocyte cells were detected in the circulating blood 2-4 days later.
format Artigo
author Grisolia, Cesar Koppe
Cordeiro, Célia Maria Torres
spellingShingle Grisolia, Cesar Koppe
Cordeiro, Célia Maria Torres
Variability in micronucleus induction with different mutagens applied to several species of fish
author_sort Grisolia, Cesar Koppe
title Variability in micronucleus induction with different mutagens applied to several species of fish
title_short Variability in micronucleus induction with different mutagens applied to several species of fish
title_full Variability in micronucleus induction with different mutagens applied to several species of fish
title_fullStr Variability in micronucleus induction with different mutagens applied to several species of fish
title_full_unstemmed Variability in micronucleus induction with different mutagens applied to several species of fish
title_sort variability in micronucleus induction with different mutagens applied to several species of fish
publisher Sociedade Brasileira de Genética
publishDate 2017
url http://repositorio.unb.br/handle/10482/25662
_version_ 1641988536211079168
score 13.657419

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