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Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction

Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase an...

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Main Authors: Britto, Constança, Silveira, Celeste Aída Nogueira, Cardoso, Maria Angelica, Marques, Patricia, Ostermayer, Alejandro Luquetti, Macêdo, Vanize de Oliveira, Fernandes, Octavio
Format: Artigo
Language: English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Subjects:
Trypanosoma cruzi
DNA de cinetoplasto
Chagas, Doença de
PCR
Tratamento
Online Access: http://repositorio.unb.br/handle/10482/25702
https://dx.doi.org/10.1590/S0074-02762001000600014
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spelling ir-10482-257022021-04-22T18:27:51Z Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction Britto, Constança Silveira, Celeste Aída Nogueira Cardoso, Maria Angelica Marques, Patricia Ostermayer, Alejandro Luquetti Macêdo, Vanize de Oliveira Fernandes, Octavio Trypanosoma cruzi DNA de cinetoplasto Chagas, Doença de PCR Tratamento Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy. 2017-12-07T04:33:49Z 2017-12-07T04:33:49Z 2001-08 Artigo BRITTO, Constança et al. Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 6, p. 823-826, ago. 2001. DOI: https://doi.org/10.1590/S0074-02762001000600014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000600014&lng=en&nrm=iso. Acesso em: 19 mar. 2021. http://repositorio.unb.br/handle/10482/25702 https://dx.doi.org/10.1590/S0074-02762001000600014 en Acesso Aberto Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000600014&lng=en&tlng=en. Acesso em: 19 mar. 2021. application/pdf Instituto Oswaldo Cruz, Ministério da Saúde
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Trypanosoma cruzi
DNA de cinetoplasto
Chagas, Doença de
PCR
Tratamento
spellingShingle Trypanosoma cruzi
DNA de cinetoplasto
Chagas, Doença de
PCR
Tratamento
Britto, Constança
Silveira, Celeste Aída Nogueira
Cardoso, Maria Angelica
Marques, Patricia
Ostermayer, Alejandro Luquetti
Macêdo, Vanize de Oliveira
Fernandes, Octavio
Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
description Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.
format Artigo
author Britto, Constança
Silveira, Celeste Aída Nogueira
Cardoso, Maria Angelica
Marques, Patricia
Ostermayer, Alejandro Luquetti
Macêdo, Vanize de Oliveira
Fernandes, Octavio
author_sort Britto, Constança
title Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_short Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_full Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_fullStr Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_full_unstemmed Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_sort parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2017
url http://repositorio.unb.br/handle/10482/25702
https://dx.doi.org/10.1590/S0074-02762001000600014
_version_ 1710449635935387648
score 13.657419

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