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Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil

Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV...

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Main Authors: FAJARDO, THOR V. M., NISHIJIMA, MARTA, BUSO, JOSÉ A., TORRES, ANTÔNIO C., ÁVILA, ANTÔNIO C., RESENDE, RENATO O.
Format: Artigo
Language: English
Published: Sociedade Brasileira de Fitopatologia 2017
Subjects:
OYDV-G
LYSV
GCLV
detection
coat protein
PCR
probes
epidemiology
Online Access: http://repositorio.unb.br/handle/10482/25724
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spelling ir-10482-257242019-03-28T14:04:07Z Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil Complexo viral do alho: identificação de Potyvirus e Carlavirus na região central do Brasil FAJARDO, THOR V. M. NISHIJIMA, MARTA BUSO, JOSÉ A. TORRES, ANTÔNIO C. ÁVILA, ANTÔNIO C. RESENDE, RENATO O. OYDV-G LYSV GCLV detection coat protein PCR probes epidemiology Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated. Infecções virais em alho são normalmente causadas por um complexo viral. Neste estudo, um complexo viral de alho, coletado em campo, foi purificado. Procedeu-se à amplificação por RT-PCR usando oligonucleotídeos desenhados para regiões-consenso dos genes das proteínas capsidiais de Onion yellow dwarf virus, estirpe do alho e de Leek yellow stripe virus. cDNA de Garlic common latent virus foi sintetizado usando oligo-dT e oligonucleotídeos aleatórios. Por estes procedimentos clones de diferentes espécies virais foram isolados e sequenciados. A análise das sequências nucleotídicas e os resultados sorológicos revelaram a presença dos Potyvirus OYDV-G e LYSV e do Carlavirus GCLV, simultaneamente infetando plantas de alho. As seqüências de aminoácidos deduzidos dos isolados brasileiros foram comparadas com aquelas de vírus relacionados, relatados em diferentes regiões do mundo. A análise mostrou pequena variabilidade em relação aos isolados brasileiros de OYDV-G e GCLV, e maior divergência em relação ao isolado de LYSV. A detecção destas espécies virais também foi obtida por reações específicas observadas quando o gene da proteína capsídica dos isolados brasileiros foi usado como sonda em ensaios de hibridização do tipo dot-blot e Southern blot. Em campo, a re-infecção natural de alho livre de vírus foi avaliada. 2017-12-07T04:33:54Z 2017-12-07T04:33:54Z 2001 Artigo Fitopatol. bras.,v.26,n.3,p.619-626,2001 0100-4158 http://repositorio.unb.br/handle/10482/25724 10.1590/S0100-41582001000300007 en Acesso Aberto application/pdf Sociedade Brasileira de Fitopatologia http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-41582001000300007&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-41582001000300007&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic OYDV-G
LYSV
GCLV
detection
coat protein
PCR
probes
epidemiology
spellingShingle OYDV-G
LYSV
GCLV
detection
coat protein
PCR
probes
epidemiology
FAJARDO, THOR V. M.
NISHIJIMA, MARTA
BUSO, JOSÉ A.
TORRES, ANTÔNIO C.
ÁVILA, ANTÔNIO C.
RESENDE, RENATO O.
Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
description Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were compared with related viruses reported in different geographical regions of the world. The analysis showed closed relations considering the Brazilian isolates of OYDV-G and GCLV, and large divergence considering LYSV isolate. The detection of these virus species was confirmed by specific reactions observed when coat protein genes of the Brazilian isolates were used as probes in dot-blot and Southern blot hybridization assays. In field natural viral re-infection of virus-free garlic was evaluated.
format Artigo
author FAJARDO, THOR V. M.
NISHIJIMA, MARTA
BUSO, JOSÉ A.
TORRES, ANTÔNIO C.
ÁVILA, ANTÔNIO C.
RESENDE, RENATO O.
author_sort FAJARDO, THOR V. M.
title Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_short Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_full Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_fullStr Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_full_unstemmed Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
title_sort garlic viral complex: identification of potyviruses and carlavirus in central brazil
publisher Sociedade Brasileira de Fitopatologia
publishDate 2017
url http://repositorio.unb.br/handle/10482/25724
_version_ 1641988520096563200
score 13.657419

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