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Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids

Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident...

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Main Authors: Azevedo, R.B., Lacava, Z.G.M., Miyasaka, C.K., Chaves, S.B., Curi, R.
Format: Artigo
Language: English
Published: Associação Brasileira de Divulgação Científica 2017
Subjects:
antioxidant enzymes
macrophages
sex hormones
estrogen
progesterone
castration
Online Access: http://repositorio.unb.br/handle/10482/25733
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spelling ir-10482-257332019-03-28T14:04:07Z Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids Azevedo, R.B. Lacava, Z.G.M. Miyasaka, C.K. Chaves, S.B. Curi, R. antioxidant enzymes macrophages sex hormones estrogen progesterone castration Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in SOD activity from 83 to 138% and reduced the difference in CAT activity from 180 to 86%. Castration and testosterone administration did not significantly modify the activities of the antioxidant enzymes in male macrophages. Ovariectomy did not affect SOD or GSH-Px activity but markedly reduced (48%) CAT activity. This latter change was fully reversed by estrogen administration, whereas progesterone had a smaller effect. These results led us to conclude that differences in the SOD and CAT activities may partially explain some of the differences in immune function reported for males and females. Also, estrogen is a potent regulator of CAT in macrophages and therefore this enzyme activity in macrophages may vary considerably during the menstrual cycle. 2017-12-07T04:33:55Z 2017-12-07T04:33:55Z 2001 Artigo Braz J Med Biol Res,v.34,n.5,p.683-687,2001 0100-879X http://repositorio.unb.br/handle/10482/25733 10.1590/S0100-879X2001000500018 en Acesso Aberto application/pdf Associação Brasileira de Divulgação Científica http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500018&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2001000500018&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-879X2001000500018&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic antioxidant enzymes
macrophages
sex hormones
estrogen
progesterone
castration
spellingShingle antioxidant enzymes
macrophages
sex hormones
estrogen
progesterone
castration
Azevedo, R.B.
Lacava, Z.G.M.
Miyasaka, C.K.
Chaves, S.B.
Curi, R.
Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
description Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in SOD activity from 83 to 138% and reduced the difference in CAT activity from 180 to 86%. Castration and testosterone administration did not significantly modify the activities of the antioxidant enzymes in male macrophages. Ovariectomy did not affect SOD or GSH-Px activity but markedly reduced (48%) CAT activity. This latter change was fully reversed by estrogen administration, whereas progesterone had a smaller effect. These results led us to conclude that differences in the SOD and CAT activities may partially explain some of the differences in immune function reported for males and females. Also, estrogen is a potent regulator of CAT in macrophages and therefore this enzyme activity in macrophages may vary considerably during the menstrual cycle.
format Artigo
author Azevedo, R.B.
Lacava, Z.G.M.
Miyasaka, C.K.
Chaves, S.B.
Curi, R.
author_sort Azevedo, R.B.
title Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
title_short Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
title_full Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
title_fullStr Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
title_full_unstemmed Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
title_sort regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
publisher Associação Brasileira de Divulgação Científica
publishDate 2017
url http://repositorio.unb.br/handle/10482/25733
_version_ 1641988266924179456
score 13.657419

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