Staff View: Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting

Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting

Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its li...

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Main Authors:	Trindade, Loiselene C., Lima, Mirtes F., Ferreira, Marisa A. S. V.
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Fitopatologia 2017
Subjects:	diversity grapevine bacterial canker Vitis vinifera repetitive DNA diversidade cancro bacteriano DNA repetitivo
Online Access:	http://repositorio.unb.br/handle/10482/26357
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spelling	ir-10482-263572019-03-28T14:05:56Z Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting Caracterização molecular de isolados brasileiros de Xanthomonas campestris pv. viticola através de rep-PCR Trindade, Loiselene C. Lima, Mirtes F. Ferreira, Marisa A. S. V. diversity grapevine bacterial canker Vitis vinifera repetitive DNA diversidade cancro bacteriano Vitis vinifera DNA repetitivo Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected. O cancro bacteriano da videira (Vitis vinifera), causado por Xanthomonas campestris pv. viticola, foi primeiramente detectado no Brasil em 1998, em parreirais no município de Petrolina, Vale do São Francisco, Pernambuco. A doença foi também observada em Juazeiro, Bahia, e mais tarde nos estados de Piauí e Ceará. Devido à sua limitada distribuição geográfica e relativamente recente detecção no Brasil, pouco se conhece sobre a biologia e diversidade do patógeno. Padrões genômicos de rep-PCR foram gerados a partir do DNA purificado de 40 isolados de X. campestris pv. viticola, coletados entre 1998 e 2001, em diferentes localidades dos estados de Pernambuco, Bahia e Piauí. A análise combinada dos padrões obtidos com os "primers" REP, ERIC e BOX, mostraram alta similaridade entre os isolados brasileiros e o isolado tipo NCPPB 2475, originário da Índia. Os isolados brasileiros apresentaram padrões similares e algumas bandas diagnósticas, presentes em todos os isolados. Os padrões foram distintos dos isolados de outros patovares e de um isolado não-patogênico obtido de folhas de videira. O polimorfismo observado entre os isolados brasileiros permitiu a diferenciação de cinco subgrupos, sem nenhuma relação com cultivar de origem, local ou época de coleta. 2017-12-07T04:42:09Z 2017-12-07T04:42:09Z 2005 Artigo Fitopatol. bras.,v.30,n.1,p.46-54,2005 0100-4158 http://repositorio.unb.br/handle/10482/26357 10.1590/S0100-41582005000100008 en Acesso Aberto application/pdf Sociedade Brasileira de Fitopatologia http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-41582005000100008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-41582005000100008&lng=en&nrm=iso
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	diversity grapevine bacterial canker Vitis vinifera repetitive DNA diversidade cancro bacteriano Vitis vinifera DNA repetitivo
spellingShingle	diversity grapevine bacterial canker Vitis vinifera repetitive DNA diversidade cancro bacteriano Vitis vinifera DNA repetitivo Trindade, Loiselene C. Lima, Mirtes F. Ferreira, Marisa A. S. V. Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
description	Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.
format	Artigo
author	Trindade, Loiselene C. Lima, Mirtes F. Ferreira, Marisa A. S. V.
author_sort	Trindade, Loiselene C.
title	Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_short	Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_full	Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_fullStr	Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_full_unstemmed	Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_sort	molecular characterization of brazilian strains of xanthomonas campestris pv. viticola by rep-pcr fingerprinting
publisher	Sociedade Brasileira de Fitopatologia
publishDate	2017
url	http://repositorio.unb.br/handle/10482/26357
_version_	1641988745384165376
score	13.657419

Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting

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