Staff View: Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil

Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil

Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3'...

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Main Authors:	Dianese, Érico de C., Ramalho, Eduardo D., Cerqueira, Daniela M., Lopes, Daniela B., Fajardo, Thor V.M., Ferreira, Marisa A.S.V., Martins, Cláudia R.F.
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Fitopatologia 2017
Subjects:	GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase
Online Access:	http://repositorio.unb.br/handle/10482/26361
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spelling	ir-10482-263612019-03-28T14:05:57Z Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil Variabilidade da extremidade 3' do gene da polimerase de isolados de Grapevine leafroll-associated virus-3 do Submédio do Vale do São Francisco Dianese, Érico de C. Ramalho, Eduardo D. Cerqueira, Daniela M. Lopes, Daniela B. Fajardo, Thor V.M. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase. A videira (Vitis spp.) é afetada por diversas viroses, dentre essas, o enrolamento da folha se destaca pela importância econômica. A doença é causada por um complexo formado por até oito vírus [Grapevine leafroll-associated virus (GLRaV) -1 ao -8]. O objetivo desse trabalho foi comparar a variabilidade da extremidade 3' do gene da polimerase de isolados de GLRaV-3, provenientes do Submédio do Vale do Rio São Francisco (Petrolina, PE) com a de outros isolados disponíveis no GenBank, incluindo um isolado da América do Norte e um da região sul do Brasil. O RNA viral foi extraído de três amostras infetadas, reagentes em teste de ELISA, e um fragmento de 340 pb foi amplificado por RT-PCR, utilizando-se oligonucleotídeos para a região do gene da polimerase viral compreendida entre os nucleotídeos 8267 a 8606. Observou-se que os três isolados da região do São Francisco, denominados Pet-1, Pet-2 e Pet-3, apresentaram 98% e 94% de similaridade com o isolado norte-americano (AF037268) com aquele do sul do Brasil (AF438411), respectivamente. A principal variação observada foi uma troca de um aminoácido da posição 2766 (F2766Y), considerando-se o genoma completo. Esses dados preliminares indicam a existência de uma variabilidade natural entre isolados de GLRaV-3 em videiras. Isso pode ser explicado pela propagação vegetativa e pelo longo ciclo da planta associados à propensão ao erro da RNA polimerase dependente de RNA. 2017-12-07T04:42:10Z 2017-12-07T04:42:10Z 2005 Artigo Fitopatol. bras.,v.30,n.2,p.173-176,2005 0100-4158 http://repositorio.unb.br/handle/10482/26361 10.1590/S0100-41582005000200012 en Acesso Aberto application/pdf Sociedade Brasileira de Fitopatologia http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-41582005000200012&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-41582005000200012&lng=en&nrm=iso
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase
spellingShingle	GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase GLRaV-3 Vitis spp. Ampelovirus RNA dependent RNA polymerase Dianese, Érico de C. Ramalho, Eduardo D. Cerqueira, Daniela M. Lopes, Daniela B. Fajardo, Thor V.M. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
description	Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.
format	Artigo
author	Dianese, Érico de C. Ramalho, Eduardo D. Cerqueira, Daniela M. Lopes, Daniela B. Fajardo, Thor V.M. Ferreira, Marisa A.S.V. Martins, Cláudia R.F.
author_sort	Dianese, Érico de C.
title	Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_short	Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_full	Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_fullStr	Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_full_unstemmed	Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_sort	variability of the 3' terminal of the polymerase gene of grapevine leafroll-associated virus 3 isolates from vale do são francisco, brazil
publisher	Sociedade Brasileira de Fitopatologia
publishDate	2017
url	http://repositorio.unb.br/handle/10482/26361
_version_	1641988505949175808
score	13.657419

Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil

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