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Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas

BACKGROUND: Osteosarcoma is a relatively uncommon malignant neoplasm and little information has been reported on its cell proliferation using Ki-67. OBJECTIVES: Evaluate histological, clinical and immunohistochemical parameters using Ki-67 labeling index (LI), correlate one to another and with follo...

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Main Authors:	Marinho, Larissa Cardoso, Patrício, Francy Reis da Silva, Jesus-Garcia Filho, Reynaldo, Petrilli, Antônio Sérgio, Odashiro, Luciana Nakao, Macedo, Carla, Alves, Maria Teresa Seixas
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia 2017
Subjects:	Osteosarcoma Ki-67 Immunohistochemistry Prognosis Osteossarcoma Imuno-histoquímica Prognóstico
Online Access:	http://repositorio.unb.br/handle/10482/26549
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spelling	ir-10482-265492019-03-28T14:06:36Z Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas Estudo clinicopatológico com avaliação do índice de proliferação celular avaliado pelo Ki-67 em osteossarcomas humanos Marinho, Larissa Cardoso Patrício, Francy Reis da Silva Jesus-Garcia Filho, Reynaldo Petrilli, Antônio Sérgio Odashiro, Luciana Nakao Macedo, Carla Alves, Maria Teresa Seixas Osteosarcoma Ki-67 Immunohistochemistry Prognosis Osteossarcoma Ki-67 Imuno-histoquímica Prognóstico BACKGROUND: Osteosarcoma is a relatively uncommon malignant neoplasm and little information has been reported on its cell proliferation using Ki-67. OBJECTIVES: Evaluate histological, clinical and immunohistochemical parameters using Ki-67 labeling index (LI), correlate one to another and with follow-up. MATERIAL AND METHOD: Fifty-seven patients with available clinicopathological data submitted to the study of cell proliferation as determined by Ki-67 expression measured by immunohistochemistry (IHC) staining using formalin-fixed paraffin embedded sections. In each sample, positive cells were quantified on at least a thousand nuclei and expressed as Ki-67 LI according to median value. RESULTS AND DISCUSSION: Non-significant correlations were observed in metastatic and non-metastatic cases when variables as surgery, tumor size, death and relapse were compared with Ki-67 LI values (cut-off of 45%). In the group of non-metastatic cases there was a direct correlation between higher values of Ki-67 index and better overall survival. Metastatic patients overall survival curve and LI high and low Ki-67 did not show significant differences. CONCLUSION: Based on our results the Ki-67 LI could be useful as a prognostic marker in patients without metastasis at diagnosis. INTRODUÇÃO: Osteossarcoma é uma neoplasia rara do tecido ósseo, tendo sido publicados poucos trabalhos na literatura que avaliaram o índice de proliferação celular Ki-67 (IPC-Ki-67) nesse tipo de sarcoma. OBJETIVO: Avaliação e comparação dos parâmetros clínicos, histológicos e de imunoexpressão do Ki-67 com a evolução clínica dos pacientes. MATERIAL E MÉTODO: Blocos de parafina de 57 casos de osteossarcomas, cujos pacientes apresentavam dados clinicopatológicos fidedignos, foram submetidos a estudo da proliferação celular através da técnica de imuno-histoquímica. Em cada amostra o IPC foi determinado pela contagem de núcleos marcados em pelo menos mil células neoplásicas. Em cada grupo de pacientes o IPC-Ki-67 foi considerado alto ou baixo, tendo como referência a mediana dos valores do Ki-67. RESULTADOS E DISCUSSÃO: Não houve correlação estatística significativa entre os valores do IPC-Ki-67 (valor de corte = 45%) dos osteossarcomas dos pacientes com ou sem metástases ao diagnóstico, em relação às variáveis óbito, recaída tumoral, tamanho do tumor e tipo de cirurgia. No grupo de pacientes sem metástases houve correlação direta entre o tempo de sobrevida e o índice de proliferação celular (isto é, o IPC-Ki-67 > 45% correlacionou-se com maior tempo de sobrevida). Nos casos de pacientes com metástases ao diagnóstico, os valores do IPC-Ki-67 não apresentaram correlação significativa com a sobrevida. CONCLUSÃO: Com base nos nossos resultados, o IPC-Ki-67 pode ser utilizado para avaliação prognóstica em pacientes com osteossarcoma sem metástases ao diagnóstico. 2017-12-07T04:43:44Z 2017-12-07T04:43:44Z 2005 Artigo J. Bras. Patol. Med. Lab.,v.41,n.6,p.419-424,2005 1676-2444 http://repositorio.unb.br/handle/10482/26549 10.1590/S1676-24442005000600008 en Acesso Aberto application/pdf Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442005000600008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1676-24442005000600008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1676-24442005000600008&lng=en&nrm=iso
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Osteosarcoma Ki-67 Immunohistochemistry Prognosis Osteossarcoma Ki-67 Imuno-histoquímica Prognóstico
spellingShingle	Osteosarcoma Ki-67 Immunohistochemistry Prognosis Osteossarcoma Ki-67 Imuno-histoquímica Prognóstico Marinho, Larissa Cardoso Patrício, Francy Reis da Silva Jesus-Garcia Filho, Reynaldo Petrilli, Antônio Sérgio Odashiro, Luciana Nakao Macedo, Carla Alves, Maria Teresa Seixas Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
description	BACKGROUND: Osteosarcoma is a relatively uncommon malignant neoplasm and little information has been reported on its cell proliferation using Ki-67. OBJECTIVES: Evaluate histological, clinical and immunohistochemical parameters using Ki-67 labeling index (LI), correlate one to another and with follow-up. MATERIAL AND METHOD: Fifty-seven patients with available clinicopathological data submitted to the study of cell proliferation as determined by Ki-67 expression measured by immunohistochemistry (IHC) staining using formalin-fixed paraffin embedded sections. In each sample, positive cells were quantified on at least a thousand nuclei and expressed as Ki-67 LI according to median value. RESULTS AND DISCUSSION: Non-significant correlations were observed in metastatic and non-metastatic cases when variables as surgery, tumor size, death and relapse were compared with Ki-67 LI values (cut-off of 45%). In the group of non-metastatic cases there was a direct correlation between higher values of Ki-67 index and better overall survival. Metastatic patients overall survival curve and LI high and low Ki-67 did not show significant differences. CONCLUSION: Based on our results the Ki-67 LI could be useful as a prognostic marker in patients without metastasis at diagnosis.
format	Artigo
author	Marinho, Larissa Cardoso Patrício, Francy Reis da Silva Jesus-Garcia Filho, Reynaldo Petrilli, Antônio Sérgio Odashiro, Luciana Nakao Macedo, Carla Alves, Maria Teresa Seixas
author_sort	Marinho, Larissa Cardoso
title	Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
title_short	Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
title_full	Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
title_fullStr	Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
title_full_unstemmed	Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas
title_sort	clinicopathologic study and ki-67 proliferative marker evaluation in human osteosarcomas
publisher	Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia
publishDate	2017
url	http://repositorio.unb.br/handle/10482/26549
_version_	1641988271210758144
score	13.657419

Clinicopathologic study and Ki-67 proliferative marker evaluation in human osteosarcomas

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