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Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil

The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to de...

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Main Authors: Borges, Ana Maria Tavares, Teixeira, José Marcus Sócrates, Costa, Paulo Sérgio Sucasas da, Giugliano, Loreny Gimenes, Fiaccadori, Fabíola Souza, Franco, Rachel de Carvalho e, Brito, Wilia Marta Elsner Diederichsen de, Leite, José Paulo Gagliardi, Cardoso, Divina das Dores de Paula
Format: Artigo
Language: English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Subjects:
calicivirus
acute gastroenteritis
reverse transcriptase
polymerase chain reaction
Brazil
Online Access: http://repositorio.unb.br/handle/10482/26609
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spelling ir-10482-266092019-03-28T14:06:48Z Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil Borges, Ana Maria Tavares Teixeira, José Marcus Sócrates Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Fiaccadori, Fabíola Souza Franco, Rachel de Carvalho e Brito, Wilia Marta Elsner Diederichsen de Leite, José Paulo Gagliardi Cardoso, Divina das Dores de Paula calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. 2017-12-07T04:44:14Z 2017-12-07T04:44:14Z 2006 Artigo Mem. Inst. Oswaldo Cruz,v.101,n.7,p.721-724,2006 0074-0276 http://repositorio.unb.br/handle/10482/26609 10.1590/S0074-02762006000700003 en Acesso Aberto application/pdf Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700003&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02762006000700003&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0074-02762006000700003&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic calicivirus
acute gastroenteritis
reverse transcriptase
polymerase chain reaction
Brazil
spellingShingle calicivirus
acute gastroenteritis
reverse transcriptase
polymerase chain reaction
Brazil
Borges, Ana Maria Tavares
Teixeira, José Marcus Sócrates
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Fiaccadori, Fabíola Souza
Franco, Rachel de Carvalho e
Brito, Wilia Marta Elsner Diederichsen de
Leite, José Paulo Gagliardi
Cardoso, Divina das Dores de Paula
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
description The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.
format Artigo
author Borges, Ana Maria Tavares
Teixeira, José Marcus Sócrates
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Fiaccadori, Fabíola Souza
Franco, Rachel de Carvalho e
Brito, Wilia Marta Elsner Diederichsen de
Leite, José Paulo Gagliardi
Cardoso, Divina das Dores de Paula
author_sort Borges, Ana Maria Tavares
title Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
title_short Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
title_full Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
title_fullStr Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
title_full_unstemmed Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
title_sort detection of calicivirus from fecal samples from children with acute gastroenteritis in the west central region of brazil
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2017
url http://repositorio.unb.br/handle/10482/26609
_version_ 1641988846258225152
score 13.657419

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