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Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil

Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in th...

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Main Authors: Fajardo, Thor V.M., Dianese, Érico C., Eiras, Marcelo, Cerqueira, Daniela M., Lopes, Daniela B., Ferreira, Marisa A.S.V., Martins, Cláudia R.F.
Format: Artigo
Language: English
Published: Sociedade Brasileira de Fitopatologia 2017
Subjects:
Closteroviridae
Ampelovirus
GLRaV-3
Online Access: http://repositorio.unb.br/handle/10482/26887
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spelling ir-10482-268872019-03-28T14:07:50Z Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil Variabilidade do gene da proteína capsidial do Grapevine leafroll-associated virus 3 no Brasil Fajardo, Thor V.M. Dianese, Érico C. Eiras, Marcelo Cerqueira, Daniela M. Lopes, Daniela B. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. Closteroviridae Ampelovirus GLRaV-3 Closteroviridae Ampelovirus GLRaV-3 Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection. O enrolamento da folha é uma doença economicamente importante que afeta videiras (Vitis spp.). Nove vírus sorologicamente distintos, Grapevine leafroll-associated virus-1 a -9, estão associados à doença. Este estudo descreve a seqüência do gene da proteína capsidial de quatro isolados do GLRaV-3 encontrados no Vale do Rio São Francisco, Nordeste do Brasil. O RNA viral foi extraído de plantas positivas em ELISA para o GLRaV-3 e o gene da proteína capsidial completo foi amplificado por RT-PCR. As seqüências foram geradas automaticamente e comparadas a seqüências completas do gene da proteína capsidial de isolados Norte-Americano (NY1) e Chineses (Dawanhong Nº;2 e SL10) de GLRaV-3. Os quatro isolados estudados, denominados Pet-1 a 4, exibiram identidades de aminoácidos deduzidos de 98-100% (Pet-1 a 3) e 95% (Pet-4) com os isolados Norte-Americano e Chineses. Um total de dezessete substituições de aminoácidos foi detectado entre os quatro isolados caracterizados em comparação com as seqüências do NY1, Dawanhong No.2 e SL10. Os resultados indicaram a existência de variação natural entre os isolados de GLRaV-3 de videiras, demonstrando também a falta de correlação entre dados de sequência e origem geográfica. Esta variabilidade deve ser considerada quando se selecionam regiões do genoma viral para uma detecção molecular confiável e consistente. 2017-12-07T04:47:27Z 2017-12-07T04:47:27Z 2007 Artigo Fitopatol. bras.,v.32,n.4,p.335-340,2007 0100-4158 http://repositorio.unb.br/handle/10482/26887 10.1590/S0100-41582007000400008 en Acesso Aberto application/pdf Sociedade Brasileira de Fitopatologia http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-41582007000400008&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-41582007000400008&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Closteroviridae
Ampelovirus
GLRaV-3
Closteroviridae
Ampelovirus
GLRaV-3
spellingShingle Closteroviridae
Ampelovirus
GLRaV-3
Closteroviridae
Ampelovirus
GLRaV-3
Fajardo, Thor V.M.
Dianese, Érico C.
Eiras, Marcelo
Cerqueira, Daniela M.
Lopes, Daniela B.
Ferreira, Marisa A.S.V.
Martins, Cláudia R.F.
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
description Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.
format Artigo
author Fajardo, Thor V.M.
Dianese, Érico C.
Eiras, Marcelo
Cerqueira, Daniela M.
Lopes, Daniela B.
Ferreira, Marisa A.S.V.
Martins, Cláudia R.F.
author_sort Fajardo, Thor V.M.
title Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_short Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_fullStr Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full_unstemmed Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_sort variability of the coat protein gene of grapevine leafroll-associated virus 3 in brazil
publisher Sociedade Brasileira de Fitopatologia
publishDate 2017
url http://repositorio.unb.br/handle/10482/26887
_version_ 1641988297932668928
score 13.657419

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