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Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)

The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary ce...

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Main Authors: Khouri, Juliana, Resck, Inês Sabioni, Fonseca, Márcio José Poças, Sousa, Thiago Machado Mello de, Pereira, Luciana Oliveira, Oliveira, Adriano Barros Barata de, Grisolia, Cesar Koppe
Format: Artigo
Language: English
Published: Sociedade Brasileira de Genética 2017
Subjects:
Pequi
Anticlastogenicidade
Antioxidantes
Micronúcleo
Online Access: http://repositorio.unb.br/handle/10482/27069
https://dx.doi.org/10.1590/S1415-47572007000300024
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spelling ir-10482-270692021-03-19T02:15:56Z Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb) Khouri, Juliana Resck, Inês Sabioni Fonseca, Márcio José Poças Sousa, Thiago Machado Mello de Pereira, Luciana Oliveira Oliveira, Adriano Barros Barata de Grisolia, Cesar Koppe Pequi Anticlastogenicidade Antioxidantes Micronúcleo The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals ( OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had been treated with the highest dose of cyclophosphamide. When CHO-K1 cells were pretreated by adding 0.01, 0.05 or 0.1 mL of extract per mL of cell culture medium 24 or 48 h before bleomycin or cyclophosphamide there was a protective effect against chromosome breaks and a significant decrease in the mitotic index (a measure of cytotoxicity) of the CHO-K1 cells. The extract also had a protective effect against oxidative hydroxyl radical damage to 2-DR. This study suggests that C. brasiliense pulp aqueous extract has anticlastogenic potential, possibly due to its antioxidative properties. 2017-12-07T04:48:28Z 2017-12-07T04:48:28Z 2007-03 Artigo KHOURI, Juliana et al. Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb). Genetics and Molecular Biology, São Paulo, v. 30, n. 2, p. 442-448, mar. 2007. DOI: https://doi.org/10.1590/S1415-47572007000300024. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024&lng=en&nrm=iso. Acesso em: 12 jan. 2021. http://repositorio.unb.br/handle/10482/27069 https://dx.doi.org/10.1590/S1415-47572007000300024 en Acesso Aberto Genetics and Molecular Biology - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024&lng=en&tlng=en. Acesso em: 12 jan. 2021. application/pdf Sociedade Brasileira de Genética
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Pequi
Anticlastogenicidade
Antioxidantes
Micronúcleo
spellingShingle Pequi
Anticlastogenicidade
Antioxidantes
Micronúcleo
Khouri, Juliana
Resck, Inês Sabioni
Fonseca, Márcio José Poças
Sousa, Thiago Machado Mello de
Pereira, Luciana Oliveira
Oliveira, Adriano Barros Barata de
Grisolia, Cesar Koppe
Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
description The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals ( OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had been treated with the highest dose of cyclophosphamide. When CHO-K1 cells were pretreated by adding 0.01, 0.05 or 0.1 mL of extract per mL of cell culture medium 24 or 48 h before bleomycin or cyclophosphamide there was a protective effect against chromosome breaks and a significant decrease in the mitotic index (a measure of cytotoxicity) of the CHO-K1 cells. The extract also had a protective effect against oxidative hydroxyl radical damage to 2-DR. This study suggests that C. brasiliense pulp aqueous extract has anticlastogenic potential, possibly due to its antioxidative properties.
format Artigo
author Khouri, Juliana
Resck, Inês Sabioni
Fonseca, Márcio José Poças
Sousa, Thiago Machado Mello de
Pereira, Luciana Oliveira
Oliveira, Adriano Barros Barata de
Grisolia, Cesar Koppe
author_sort Khouri, Juliana
title Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
title_short Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
title_full Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
title_fullStr Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
title_full_unstemmed Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
title_sort anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (caryocar brasiliense camb)
publisher Sociedade Brasileira de Genética
publishDate 2017
url http://repositorio.unb.br/handle/10482/27069
https://dx.doi.org/10.1590/S1415-47572007000300024
_version_ 1695119543029989376
score 13.657419

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