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Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil

This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An a...

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Main Authors: Silva, Daniela de Melo e, Cruz, Aparecido Divino da, Bastos, Rogério Pereira, Reis, Raquel Loren, Telles, Mariana Pires de Campos, Diniz-Filho, José Alexandre Felizola
Format: Artigo
Language: English
Published: Sociedade Brasileira de Genética 2017
Subjects:
Eupemphix nattereri
gene flow
population structure
RAPD markers
Online Access: http://repositorio.unb.br/handle/10482/27070
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spelling ir-10482-270702019-03-28T14:08:19Z Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil Silva, Daniela de Melo e Cruz, Aparecido Divino da Bastos, Rogério Pereira Reis, Raquel Loren Telles, Mariana Pires de Campos Diniz-Filho, José Alexandre Felizola Eupemphix nattereri gene flow population structure RAPD markers This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise phist among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with averages (UPGMA) dendrogram. To determine if genetic distances were correlated to geographical distances we constructed pairwise genetic distance and geographical distance matrices and compared them using the Mantel test. The AMOVA results indicated significant genetic differences (p < 0.001) between E. nattereri populations, representing 69.5% of the within population genetic diversity. The Mantel test showed no significant correlation (r = 0.03; p = 0.45) between the genetic and geographical distance matrices. Our findings indicate that the genetic variation of E. nattereri populations was randomly distributed in geographic space and that gene flow for this species is probably structured at spatial scales smaller than those between our samples 2017-12-07T04:48:28Z 2017-12-07T04:48:28Z 2007 Artigo Genet. Mol. Biol.,v.30,n.4,p.1161-1168,2007 1415-4757 http://repositorio.unb.br/handle/10482/27070 10.1590/S1415-47572007000600022 en Acesso Aberto application/pdf Sociedade Brasileira de Genética http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600022&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572007000600022&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1415-47572007000600022&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Eupemphix nattereri
gene flow
population structure
RAPD markers
spellingShingle Eupemphix nattereri
gene flow
population structure
RAPD markers
Silva, Daniela de Melo e
Cruz, Aparecido Divino da
Bastos, Rogério Pereira
Reis, Raquel Loren
Telles, Mariana Pires de Campos
Diniz-Filho, José Alexandre Felizola
Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
description This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise phist among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with averages (UPGMA) dendrogram. To determine if genetic distances were correlated to geographical distances we constructed pairwise genetic distance and geographical distance matrices and compared them using the Mantel test. The AMOVA results indicated significant genetic differences (p < 0.001) between E. nattereri populations, representing 69.5% of the within population genetic diversity. The Mantel test showed no significant correlation (r = 0.03; p = 0.45) between the genetic and geographical distance matrices. Our findings indicate that the genetic variation of E. nattereri populations was randomly distributed in geographic space and that gene flow for this species is probably structured at spatial scales smaller than those between our samples
format Artigo
author Silva, Daniela de Melo e
Cruz, Aparecido Divino da
Bastos, Rogério Pereira
Reis, Raquel Loren
Telles, Mariana Pires de Campos
Diniz-Filho, José Alexandre Felizola
author_sort Silva, Daniela de Melo e
title Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
title_short Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
title_full Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
title_fullStr Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
title_full_unstemmed Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
title_sort population structure of eupemphix nattereri (amphibia, anura, leiuperidae) from central brazil
publisher Sociedade Brasileira de Genética
publishDate 2017
url http://repositorio.unb.br/handle/10482/27070
_version_ 1641988345939623936
score 13.657419

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