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Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp

Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish...

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Main Authors: Silva, Ricardo Rocha Pavan da, Pires Junior, Osmindo Rodrigues, Grisolia, Cesar Koppe
Format: Artigo
Language: English
Published: Sociedade Brasileira de Genética 2017
Subjects:
Peixe
Necrose
Apoptose
Micronúcleo
Genotoxicidade
Online Access: http://repositorio.unb.br/handle/10482/27852
https://dx.doi.org/10.1590/S1415-47572010000400023
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spelling ir-10482-278522021-04-22T14:08:04Z Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp Silva, Ricardo Rocha Pavan da Pires Junior, Osmindo Rodrigues Grisolia, Cesar Koppe Peixe Necrose Apoptose Micronúcleo Genotoxicidade Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish Astyanax bimaculatus, a native species from South America. LC50 (72 h) was determined as 242.81 µg L-1 and LD50 (72 h) as 49.19 µg kg-1 bw. There was a significant increase of DNA damage in peripheral erythrocytes, following intraperitoneal injection (ip) with tested concentrations of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw, as well as through body exposure to a concentration of 103.72 µg L-1. Micronucleus (MN) induction was observed after ip injections of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw for 72 h, as well as following body exposure for 72 at 103.72 µg L-1. Thus, both exposure routes resulted in MN induction and DNA damage. Apoptosis-necrosis testing was carried out only by ip injection with concentrations of 24.58 µg -1 bw and 36.88 µg kg-1 bw. Exposure to microcystins at lower concentrations induced more apoptosis than necrosis in peripheral erythrocytes, whereas exposure at higher concentrations gave rise to both conditions. Thus, Astyanax bimaculatus can be considered as a species sensitive to the genotoxic effects caused by microcystins. 2017-12-07T04:54:34Z 2017-12-07T04:54:34Z 2010 Artigo SILVA, Ricardo Rocha Pavan da; PIRES JUNIOR, Osmindo Rodrigues; GRISOLIA, Cesar Koppe. Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp. Genetics and Molecular Biology, São Paulo, v. 33, n. 4, p. 750-755, 2010. DOI: http://dx.doi.org/10.1590/S1415-47572010000400023. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400023&lng=en&nrm=iso. Acesso em: 19 mar. 2021. http://repositorio.unb.br/handle/10482/27852 https://dx.doi.org/10.1590/S1415-47572010000400023 en Acesso Aberto Genetics and Molecular Biology - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400023&lng=en&tlng=en. Acesso em: 19 mar. 2021 application/pdf Sociedade Brasileira de Genética
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Peixe
Necrose
Apoptose
Micronúcleo
Genotoxicidade
spellingShingle Peixe
Necrose
Apoptose
Micronúcleo
Genotoxicidade
Silva, Ricardo Rocha Pavan da
Pires Junior, Osmindo Rodrigues
Grisolia, Cesar Koppe
Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
description Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish Astyanax bimaculatus, a native species from South America. LC50 (72 h) was determined as 242.81 µg L-1 and LD50 (72 h) as 49.19 µg kg-1 bw. There was a significant increase of DNA damage in peripheral erythrocytes, following intraperitoneal injection (ip) with tested concentrations of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw, as well as through body exposure to a concentration of 103.72 µg L-1. Micronucleus (MN) induction was observed after ip injections of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw for 72 h, as well as following body exposure for 72 at 103.72 µg L-1. Thus, both exposure routes resulted in MN induction and DNA damage. Apoptosis-necrosis testing was carried out only by ip injection with concentrations of 24.58 µg -1 bw and 36.88 µg kg-1 bw. Exposure to microcystins at lower concentrations induced more apoptosis than necrosis in peripheral erythrocytes, whereas exposure at higher concentrations gave rise to both conditions. Thus, Astyanax bimaculatus can be considered as a species sensitive to the genotoxic effects caused by microcystins.
format Artigo
author Silva, Ricardo Rocha Pavan da
Pires Junior, Osmindo Rodrigues
Grisolia, Cesar Koppe
author_sort Silva, Ricardo Rocha Pavan da
title Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
title_short Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
title_full Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
title_fullStr Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
title_full_unstemmed Toxicity and genotoxicity in astyanax bimaculatus (Characidae) induced by microcystins from a bloom of microcystis spp
title_sort toxicity and genotoxicity in astyanax bimaculatus (characidae) induced by microcystins from a bloom of microcystis spp
publisher Sociedade Brasileira de Genética
publishDate 2017
url http://repositorio.unb.br/handle/10482/27852
https://dx.doi.org/10.1590/S1415-47572010000400023
_version_ 1701480382438834176
score 13.657419

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