Cover Image

Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction

This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutinat...

Full description

Main Authors: Vieira, Anahí Souto, Rosinha, Grácia Maria Soares, Oliveira, Carina Elisei de, Vasconcellos, Silvio Arruda, Lima-Borges, Paulo Andre, Tomás, Walfrido Moraes, Mourão, Guilherme Miranda, Lacerda, Ana Cristina Reis, Soares, Cleber Oliveira, Araújo, Flábio Ribeiro de, Piovezan, Ubiratan, Zucco, Carlos Andre, Pellegrin, Aiesca Oliveira
Format: Artigo
Language: English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Subjects:
pampas deer
Leptospira spp
MAT
PCR
Pantanal
Online Access: http://repositorio.unb.br/handle/10482/27973
Tags: Add Tag
No Tags, Be the first to tag this record!
id ir-10482-27973
recordtype dspace
spelling ir-10482-279732019-03-28T14:02:29Z Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction Vieira, Anahí Souto Rosinha, Grácia Maria Soares Oliveira, Carina Elisei de Vasconcellos, Silvio Arruda Lima-Borges, Paulo Andre Tomás, Walfrido Moraes Mourão, Guilherme Miranda Lacerda, Ana Cristina Reis Soares, Cleber Oliveira Araújo, Flábio Ribeiro de Piovezan, Ubiratan Zucco, Carlos Andre Pellegrin, Aiesca Oliveira pampas deer Leptospira spp MAT PCR Pantanal This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed. 2017-12-07T04:56:11Z 2017-12-07T04:56:11Z 2011 Artigo Mem. Inst. Oswaldo Cruz,v.106,n.6,p.763-768,2011 0074-0276 http://repositorio.unb.br/handle/10482/27973 10.1590/S0074-02762011000600019 en Acesso Aberto application/pdf Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600019&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02762011000600019&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0074-02762011000600019&lng=en&nrm=iso
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic pampas deer
Leptospira spp
MAT
PCR
Pantanal
spellingShingle pampas deer
Leptospira spp
MAT
PCR
Pantanal
Vieira, Anahí Souto
Rosinha, Grácia Maria Soares
Oliveira, Carina Elisei de
Vasconcellos, Silvio Arruda
Lima-Borges, Paulo Andre
Tomás, Walfrido Moraes
Mourão, Guilherme Miranda
Lacerda, Ana Cristina Reis
Soares, Cleber Oliveira
Araújo, Flábio Ribeiro de
Piovezan, Ubiratan
Zucco, Carlos Andre
Pellegrin, Aiesca Oliveira
Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
description This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.
format Artigo
author Vieira, Anahí Souto
Rosinha, Grácia Maria Soares
Oliveira, Carina Elisei de
Vasconcellos, Silvio Arruda
Lima-Borges, Paulo Andre
Tomás, Walfrido Moraes
Mourão, Guilherme Miranda
Lacerda, Ana Cristina Reis
Soares, Cleber Oliveira
Araújo, Flábio Ribeiro de
Piovezan, Ubiratan
Zucco, Carlos Andre
Pellegrin, Aiesca Oliveira
author_sort Vieira, Anahí Souto
title Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
title_short Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
title_full Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
title_fullStr Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
title_full_unstemmed Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
title_sort survey of leptospira spp in pampas deer (ozotoceros bezoarticus) in the pantanal wetlands of the state of mato grosso do sul, brazil by serology and polymerase chain reaction
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2017
url http://repositorio.unb.br/handle/10482/27973
_version_ 1641988589439942656
score 13.657419

Similar Items