Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women
We investigated the effect of -174 G/C single-nucleotide polymorphism in the promoter region of the IL6 gene on plasma IL-6 levels and muscle strength, and the relationship between IL-6 levels and muscle strength in elderly women. The sample consisted of 199 elderly residents (73.0 ± 7.8 years old)...
| Main Authors: | Pereira, D.S., Garcia, D.M., Narciso, F.M.S., Santos, M.L.A.S., Dias, J.M.D., Queiroz, B.Z., Souza, E.R., Nóbrega, O.T., Pereira, L.S.M. |
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| Language: | English |
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Associação Brasileira de Divulgação Científica
2017
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ir-10482-280122019-03-28T14:02:32Z Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women Pereira, D.S. Garcia, D.M. Narciso, F.M.S. Santos, M.L.A.S. Dias, J.M.D. Queiroz, B.Z. Souza, E.R. Nóbrega, O.T. Pereira, L.S.M. Interleukin-6 Elderly Muscular strength 174 G/C polymorphism We investigated the effect of -174 G/C single-nucleotide polymorphism in the promoter region of the IL6 gene on plasma IL-6 levels and muscle strength, and the relationship between IL-6 levels and muscle strength in elderly women. The sample consisted of 199 elderly residents (73.0 ± 7.8 years old) from rest homes and the community in Belo Horizonte, MG, Brazil. -174 G/C polymorphism was determined by direct sequencing of the product by PCR, and plasma IL-6 concentrations were measured by ELISA. Muscle strength in the knee joint was evaluated using a Biodex System 3 Pro® isokinetic dynamometer. ANCOVA was used to determine the effect of polymorphism on IL-6 levels and muscle strength, and the Pearson correlation coefficient to assess the relationship between IL-6 levels and muscle strength. -174 G/C polymorphism was associated with the plasma IL-6 levels of elderly women (P < 0.01) since homozygotes for the G allele showed high IL-6 levels (GG 3.85 pg/mL, GC + CC 2.13 pg/mL). There was no association of polymorphism on muscle strength (P > 0.05). No association was found between IL-6 levels and knee extensor muscle (r = 0.087, P = 0.306) or flexor (r = -0.011, P = 0.894) strength. An interaction between -174 G/C polymorphism and housing conditions of the sample of elderly women was identified, with the effect of genotype on IL-6 levels being higher in the institutionalized elderly. These results support the evidence that -174 G/C polymorphism of the IL6 gene associates with individual variability of plasma IL-6 levels in elderly women. 2017-12-07T04:56:19Z 2017-12-07T04:56:19Z 2011 Artigo Braz J Med Biol Res,v.44,n.2,p.123-129,2011 0100-879X http://repositorio.unb.br/handle/10482/28012 10.1590/S0100-879X2010007500152 en Acesso Aberto application/pdf Associação Brasileira de Divulgação Científica http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200006&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2011000200006&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-879X2011000200006&lng=en&nrm=iso |
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English |
| topic |
Interleukin-6 Elderly Muscular strength 174 G/C polymorphism |
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Interleukin-6 Elderly Muscular strength 174 G/C polymorphism Pereira, D.S. Garcia, D.M. Narciso, F.M.S. Santos, M.L.A.S. Dias, J.M.D. Queiroz, B.Z. Souza, E.R. Nóbrega, O.T. Pereira, L.S.M. Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| description |
We investigated the effect of -174 G/C single-nucleotide polymorphism in the promoter region of the IL6 gene on plasma IL-6 levels and muscle strength, and the relationship between IL-6 levels and muscle strength in elderly women. The sample consisted of 199 elderly residents (73.0 ± 7.8 years old) from rest homes and the community in Belo Horizonte, MG, Brazil. -174 G/C polymorphism was determined by direct sequencing of the product by PCR, and plasma IL-6 concentrations were measured by ELISA. Muscle strength in the knee joint was evaluated using a Biodex System 3 Pro® isokinetic dynamometer. ANCOVA was used to determine the effect of polymorphism on IL-6 levels and muscle strength, and the Pearson correlation coefficient to assess the relationship between IL-6 levels and muscle strength. -174 G/C polymorphism was associated with the plasma IL-6 levels of elderly women (P < 0.01) since homozygotes for the G allele showed high IL-6 levels (GG 3.85 pg/mL, GC + CC 2.13 pg/mL). There was no association of polymorphism on muscle strength (P > 0.05). No association was found between IL-6 levels and knee extensor muscle (r = 0.087, P = 0.306) or flexor (r = -0.011, P = 0.894) strength. An interaction between -174 G/C polymorphism and housing conditions of the sample of elderly women was identified, with the effect of genotype on IL-6 levels being higher in the institutionalized elderly. These results support the evidence that -174 G/C polymorphism of the IL6 gene associates with individual variability of plasma IL-6 levels in elderly women. |
| format |
Artigo |
| author |
Pereira, D.S. Garcia, D.M. Narciso, F.M.S. Santos, M.L.A.S. Dias, J.M.D. Queiroz, B.Z. Souza, E.R. Nóbrega, O.T. Pereira, L.S.M. |
| author_sort |
Pereira, D.S. |
| title |
Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| title_short |
Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| title_full |
Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| title_fullStr |
Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| title_full_unstemmed |
Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women |
| title_sort |
effects of 174 g/c polymorphism in the promoter region of the interleukin-6 gene on plasma il-6 levels and muscle strength in elderly women |
| publisher |
Associação Brasileira de Divulgação Científica |
| publishDate |
2017 |
| url |
http://repositorio.unb.br/handle/10482/28012 |
| _version_ |
1641988222725652480 |
| score |
13.657419 |