Staff View: Limb immobilization alters functional electrophysiological parameters of sciatic nerve

Limb immobilization alters functional electrophysiological parameters of sciatic nerve

Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic n...

Full description

Main Authors:	Alves, J. S. M., Leal-Cardoso, J. H., Santos-Júnior, F. F. U., Carlos, P. S., Silva, Renata Carvalho, Lucci, Carolina Madeira, Báo, Sônia Nair, Ceccatto, V. M., Barbosa, R.
Format:	Artigo
Language:	English
Published:	Associação Brasileira de Divulgação Científica 2017
Subjects:	Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia
Online Access:	http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626
Tags:	Add Tag No Tags, Be the first to tag this record!

id	ir-10482-28739
recordtype	dspace
spelling	ir-10482-287392021-01-27T18:38:47Z Limb immobilization alters functional electrophysiological parameters of sciatic nerve Alves, J. S. M. Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R. Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function. 2017-12-07T05:02:34Z 2017-12-07T05:02:34Z 2013-08 Artigo ALVES, J. S. M. et al. Limb immobilization alters functional electrophysiological parameters of sciatic nerve. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 8, p. 715-721, ago. 2013. DOI: https://doi.org/10.1590/1414-431X20132626. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&nrm=iso. Acesso em: 04 dez. 2020. http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626 en Acesso Aberto Brazilian Journal of Medical and Biological Research - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&tlng=en. Acesso em: 04 dez. 2020. application/pdf Associação Brasileira de Divulgação Científica
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia
spellingShingle	Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia Alves, J. S. M. Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R. Limb immobilization alters functional electrophysiological parameters of sciatic nerve
description	Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function.
format	Artigo
author	Alves, J. S. M. Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R.
author_sort	Alves, J. S. M.
title	Limb immobilization alters functional electrophysiological parameters of sciatic nerve
title_short	Limb immobilization alters functional electrophysiological parameters of sciatic nerve
title_full	Limb immobilization alters functional electrophysiological parameters of sciatic nerve
title_fullStr	Limb immobilization alters functional electrophysiological parameters of sciatic nerve
title_full_unstemmed	Limb immobilization alters functional electrophysiological parameters of sciatic nerve
title_sort	limb immobilization alters functional electrophysiological parameters of sciatic nerve
publisher	Associação Brasileira de Divulgação Científica
publishDate	2017
url	http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626
_version_	1690679525876170752
score	13.657419

Limb immobilization alters functional electrophysiological parameters of sciatic nerve

Similar Items