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Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis

The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studie...

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Main Authors: Almeida, Richardson Alves de, Gordo, Marcelo, Silva, Felipe Moura Araujo da, Araújo, Rafael C. de, Ramada, Marcelo Henrique Soller, Abrão, Fernando Yano, Costa, Túlio O. G., Koolen, Hector Henrique Ferreira, Souza, Afonso Duarte Leão de, Bloch Júnior, Carlos
Format: Artigo
Language: English
Published: Sociedade Brasileira de Química 2017
Subjects:
Anuro
Peptídeos antimicrobianos
Secreção cutânea
Online Access: http://repositorio.unb.br/handle/10482/29693
http://dx.doi.org/10.5935/0103-5053.20150219
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spelling ir-10482-296932019-03-28T14:08:26Z Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis Almeida, Richardson Alves de Gordo, Marcelo Silva, Felipe Moura Araujo da Araújo, Rafael C. de Ramada, Marcelo Henrique Soller Abrão, Fernando Yano Costa, Túlio O. G. Koolen, Hector Henrique Ferreira Souza, Afonso Duarte Leão de Bloch Júnior, Carlos Anuro Peptídeos antimicrobianos Secreção cutânea The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM. 2017-12-07T05:12:55Z 2017-12-07T05:12:55Z 2015-11 Artigo ALMEIDA, Richardson Alves de et al. Cinerascetins, new peptides from Hypsiboas cinerascens: MALDI LIFT-TOF-MS/MS de novo sequence and imaging snalysis. Journal of the Brazilian Chemical Society, São Paulo, v. 26, n. 11, p. 2290-2297, nov. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001102290&lng=en&nrm=iso>. Acesso em: 16 abr. 2018. doi: http://dx.doi.org/10.5935/0103-5053.20150219. http://repositorio.unb.br/handle/10482/29693 http://dx.doi.org/10.5935/0103-5053.20150219 en Acesso Aberto Journal of the Brazilian Chemical Society - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001102290&lng=en&nrm=iso. Acesso em: 16 abr. 2018. application/pdf Sociedade Brasileira de Química
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language English
topic Anuro
Peptídeos antimicrobianos
Secreção cutânea
spellingShingle Anuro
Peptídeos antimicrobianos
Secreção cutânea
Almeida, Richardson Alves de
Gordo, Marcelo
Silva, Felipe Moura Araujo da
Araújo, Rafael C. de
Ramada, Marcelo Henrique Soller
Abrão, Fernando Yano
Costa, Túlio O. G.
Koolen, Hector Henrique Ferreira
Souza, Afonso Duarte Leão de
Bloch Júnior, Carlos
Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
description The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.
format Artigo
author Almeida, Richardson Alves de
Gordo, Marcelo
Silva, Felipe Moura Araujo da
Araújo, Rafael C. de
Ramada, Marcelo Henrique Soller
Abrão, Fernando Yano
Costa, Túlio O. G.
Koolen, Hector Henrique Ferreira
Souza, Afonso Duarte Leão de
Bloch Júnior, Carlos
author_sort Almeida, Richardson Alves de
title Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
title_short Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
title_full Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
title_fullStr Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
title_full_unstemmed Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
title_sort cinerascetins, new peptides from hypsiboas cinerascens : maldi lift-tof-ms/ms de novo sequence and imaging analysis
publisher Sociedade Brasileira de Química
publishDate 2017
url http://repositorio.unb.br/handle/10482/29693
http://dx.doi.org/10.5935/0103-5053.20150219
_version_ 1641988391462502400
score 13.657419

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