Staff View: Produção de embriões em ovinos Morada Nova e Somalis Brasileira

Produção de embriões em ovinos Morada Nova e Somalis Brasileira

Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR(r)) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg...

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Main Authors:	Brasil, Oscar Oliveira, Moreira, Nathalia Hack, Silva, Thiago Antonio Souza Nascimento, Silva, Bianca Damiani Marques, Nascimento, Normandes Vieira, Facó, Olivardo, Ramos, Alexandre Floriani
Format:	Artigo
Language:	Portuguese
Published:	Universidade Federal de Minas Gerais, Escola de Veterinária 2017
Subjects:	Ovulação Embrião Ovino - reprodução
Online Access:	http://repositorio.unb.br/handle/10482/30103 http://dx.doi.org/10.1590/1678-4162-8428
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spelling	ir-10482-301032018-11-30T10:43:55Z Produção de embriões em ovinos Morada Nova e Somalis Brasileira Embryo production in Morada Nova and Somalis Brasileira ewes Brasil, Oscar Oliveira Moreira, Nathalia Hack Silva, Thiago Antonio Souza Nascimento Silva, Bianca Damiani Marques Nascimento, Normandes Vieira Facó, Olivardo Ramos, Alexandre Floriani Ovulação Embrião Ovino - reprodução Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR(r)) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment, and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR removal). Fifty hours after CIDR(r) removal, females were inseminated by laparoscopy. All embryos were recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation protocol (P>0.05) included 74% of the Morada Nova ewes and 50% of the Somalis Brasileira ewes. Morada Nova showed better results (P<0.05) than Somalis Brasileira in number of ovulations (15.38 ± 5.24 vs. 10.56 ± 2.83), total structures (11.00 ± 7.55 vs. 3.33 ± 1.94) and embryo yields (6.79 ± 5.35 vs. 2.90 ± 2.18). Despite the high fertilization rate, degenerate embryo rate was high for both breeds, with an overall rate of 39% (57/145). In conclusion, superovulatory response and embryo yields in Morada Nova ewes were considered sufficient to justify the use of this procedure in genetic resources conservation programs. However, improvements to embryo quality and control of precocious regression of corpus luteum are necessary to produce better results in the MOET program, with minimal variations and maximum embryo yield in Morada Nova and Somalis Brasileira ewes. 2017-12-07T05:17:42Z 2017-12-07T05:17:42Z 2016-09 Artigo BRASIL, O.O. et al. Produção de embriões em ovinos Morada Nova e Somalis Brasileira. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 68, n. 5, p. 1390-1394, set./out. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501390&lng=en&nrm=iso>. Acesso em: 9 mar. 2018. doi: http://dx.doi.org/10.1590/1678-4162-8428. http://repositorio.unb.br/handle/10482/30103 http://dx.doi.org/10.1590/1678-4162-8428 pt Acesso Aberto Arquivo Brasileiro de Medicina Veterinária e Zootecnia - Este é um artigo publicado em acesso aberto sob uma licença Creative Commons (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501390&lng=en&nrm=iso. Acesso em: 9 mar. 2018. application/pdf Universidade Federal de Minas Gerais, Escola de Veterinária
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	Portuguese
topic	Ovulação Embrião Ovino - reprodução
spellingShingle	Ovulação Embrião Ovino - reprodução Brasil, Oscar Oliveira Moreira, Nathalia Hack Silva, Thiago Antonio Souza Nascimento Silva, Bianca Damiani Marques Nascimento, Normandes Vieira Facó, Olivardo Ramos, Alexandre Floriani Produção de embriões em ovinos Morada Nova e Somalis Brasileira
description	Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR(r)) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment, and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR removal). Fifty hours after CIDR(r) removal, females were inseminated by laparoscopy. All embryos were recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation protocol (P>0.05) included 74% of the Morada Nova ewes and 50% of the Somalis Brasileira ewes. Morada Nova showed better results (P<0.05) than Somalis Brasileira in number of ovulations (15.38 ± 5.24 vs. 10.56 ± 2.83), total structures (11.00 ± 7.55 vs. 3.33 ± 1.94) and embryo yields (6.79 ± 5.35 vs. 2.90 ± 2.18). Despite the high fertilization rate, degenerate embryo rate was high for both breeds, with an overall rate of 39% (57/145). In conclusion, superovulatory response and embryo yields in Morada Nova ewes were considered sufficient to justify the use of this procedure in genetic resources conservation programs. However, improvements to embryo quality and control of precocious regression of corpus luteum are necessary to produce better results in the MOET program, with minimal variations and maximum embryo yield in Morada Nova and Somalis Brasileira ewes.
format	Artigo
author	Brasil, Oscar Oliveira Moreira, Nathalia Hack Silva, Thiago Antonio Souza Nascimento Silva, Bianca Damiani Marques Nascimento, Normandes Vieira Facó, Olivardo Ramos, Alexandre Floriani
author_sort	Brasil, Oscar Oliveira
title	Produção de embriões em ovinos Morada Nova e Somalis Brasileira
title_short	Produção de embriões em ovinos Morada Nova e Somalis Brasileira
title_full	Produção de embriões em ovinos Morada Nova e Somalis Brasileira
title_fullStr	Produção de embriões em ovinos Morada Nova e Somalis Brasileira
title_full_unstemmed	Produção de embriões em ovinos Morada Nova e Somalis Brasileira
title_sort	produção de embriões em ovinos morada nova e somalis brasileira
publisher	Universidade Federal de Minas Gerais, Escola de Veterinária
publishDate	2017
url	http://repositorio.unb.br/handle/10482/30103 http://dx.doi.org/10.1590/1678-4162-8428
_version_	1641987973293539328
score	13.657419

Produção de embriões em ovinos Morada Nova e Somalis Brasileira

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