Staff View: Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells

Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells

Glioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal proper...

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Main Authors:	Pavon, Lorena Favaro, Sibov, Tatiana Tais, Oliveira, Daniela Mara de, Marti, Luciana C., Cabral, Francisco Romero, Souza, Jean Gabriel de, Boufleur, Pamela, Malheiros, Suzana M. F., Neto, Manuel A. de Paiva, Cruz, Edgard Ferreira da, Tavassi, Ana Marisa Chudzinski, Cavalheiro, Sérgio
Format:	Artigo
Language:	Inglês
Published:	Impact Journals 2018
Subjects:	Neurosferas Glicoproteínas Células-tronco Microscopia eletrônica Tumores
Online Access:	http://repositorio.unb.br/handle/10482/33159 https://doi.org/10.18632/oncotarget.9658
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spelling	ir-10482-331592018-12-11T12:43:37Z Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells Pavon, Lorena Favaro Sibov, Tatiana Tais Oliveira, Daniela Mara de Marti, Luciana C. Cabral, Francisco Romero Souza, Jean Gabriel de Boufleur, Pamela Malheiros, Suzana M. F. Neto, Manuel A. de Paiva Cruz, Edgard Ferreira da Tavassi, Ana Marisa Chudzinski Cavalheiro, Sérgio Neurosferas Glicoproteínas Células-tronco Microscopia eletrônica Tumores Glioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal properties of primary highly purified human CD133+ glioblastoma-initiating cells. To accomplish this aim, we used the following approaches: i) generation of tumor subspheres of CD133+ selected cells from primary cell cultures of glioblastoma; ii) analysis of the expression of pluripotency stem cell markers and mesenchymal stem cell (MSC) markers in the CD133+ glioblastoma-initiating cells; iii) side-by-side ultrastructural characterization of the CD133+ glioblastoma cells, MSC and CD133+ hematopoietic stem cells isolated from human umbilical cord blood (UCB); iv) assessment of adipogenic differentiation of CD133+ glioblastoma cells to test their MSC-like in vitro differentiation ability; and v) use of an orthotopic glioblastoma xenograft model in the absence of immune suppression. We found that the CD133+ glioblastoma cells expressed both the pluripotency stem cell markers (Nanog, Mush-1 and SSEA-3) and MSC markers. In addition, the CD133+ cells were able to differentiate into adipocyte-like cells. Transmission electron microscopy (TEM) demonstrated that the CD133+ glioblastoma-initiating cells had ultrastructural features similar to those of undifferentiated MSCs. In addition, when administered in vivo to non-immunocompromised animals, the CD133+ cells were also able to mimic the phenotype of the original patient’s tumor. In summary, we showed that the CD133+ glioblastoma cells express molecular signatures of MSCs, neural stem cells and pluripotent stem cells, thus possibly enabling differentiation into both neural and mesodermal cell types. 2018-12-11T11:01:30Z 2018-12-11T11:01:30Z 2016-05 Artigo PAVON, Lorena Favaro et al. Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells. Oncotarget, Nova York, v. 7, p. 40546-40557, maio 2016. DOI: https://doi.org/10.18632/oncotarget.9658. Disponível em: http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9658&path[]=41659. Acesso em: 11 dez. 2018. http://repositorio.unb.br/handle/10482/33159 https://doi.org/10.18632/oncotarget.9658 Inglês Acesso Aberto Oncotarget - All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License (CC BY). Fonte: http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=9658&path[]=41659. Acesso em: 11 dez. 2018. application/pdf Impact Journals
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	Inglês
topic	Neurosferas Glicoproteínas Células-tronco Microscopia eletrônica Tumores
spellingShingle	Neurosferas Glicoproteínas Células-tronco Microscopia eletrônica Tumores Pavon, Lorena Favaro Sibov, Tatiana Tais Oliveira, Daniela Mara de Marti, Luciana C. Cabral, Francisco Romero Souza, Jean Gabriel de Boufleur, Pamela Malheiros, Suzana M. F. Neto, Manuel A. de Paiva Cruz, Edgard Ferreira da Tavassi, Ana Marisa Chudzinski Cavalheiro, Sérgio Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
description	Glioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal properties of primary highly purified human CD133+ glioblastoma-initiating cells. To accomplish this aim, we used the following approaches: i) generation of tumor subspheres of CD133+ selected cells from primary cell cultures of glioblastoma; ii) analysis of the expression of pluripotency stem cell markers and mesenchymal stem cell (MSC) markers in the CD133+ glioblastoma-initiating cells; iii) side-by-side ultrastructural characterization of the CD133+ glioblastoma cells, MSC and CD133+ hematopoietic stem cells isolated from human umbilical cord blood (UCB); iv) assessment of adipogenic differentiation of CD133+ glioblastoma cells to test their MSC-like in vitro differentiation ability; and v) use of an orthotopic glioblastoma xenograft model in the absence of immune suppression. We found that the CD133+ glioblastoma cells expressed both the pluripotency stem cell markers (Nanog, Mush-1 and SSEA-3) and MSC markers. In addition, the CD133+ cells were able to differentiate into adipocyte-like cells. Transmission electron microscopy (TEM) demonstrated that the CD133+ glioblastoma-initiating cells had ultrastructural features similar to those of undifferentiated MSCs. In addition, when administered in vivo to non-immunocompromised animals, the CD133+ cells were also able to mimic the phenotype of the original patient’s tumor. In summary, we showed that the CD133+ glioblastoma cells express molecular signatures of MSCs, neural stem cells and pluripotent stem cells, thus possibly enabling differentiation into both neural and mesodermal cell types.
format	Artigo
author	Pavon, Lorena Favaro Sibov, Tatiana Tais Oliveira, Daniela Mara de Marti, Luciana C. Cabral, Francisco Romero Souza, Jean Gabriel de Boufleur, Pamela Malheiros, Suzana M. F. Neto, Manuel A. de Paiva Cruz, Edgard Ferreira da Tavassi, Ana Marisa Chudzinski Cavalheiro, Sérgio
author_sort	Pavon, Lorena Favaro
title	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
title_short	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
title_full	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
title_fullStr	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
title_full_unstemmed	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
title_sort	mesenchymal stem cell-like properties of cd133+ glioblastoma initiating cells
publisher	Impact Journals
publishDate	2018
url	http://repositorio.unb.br/handle/10482/33159 https://doi.org/10.18632/oncotarget.9658
_version_	1641987986248695808
score	13.657419

Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells

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