Staff View: The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rD...

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Main Authors:	Alves, Daniella de Sousa Mendes Moreira, Gonçalves, Gustavo Souza, Moraes, Aline Silva, Alves, Luciano Moreira, Carmo Neto, José Rodrigues do, Hecht, Mariana Machado, Nitz, Nadjar, Gonçalves, Rodrigo Gurgel, Bernardes, Geisa, Castro, Ana Maria de, Chalita, Maria Regina, Vinaud, Marina Clare
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 2019
Subjects:	Córnea - doenças Córnea - exames
Online Access:	http://repositorio.unb.br/handle/10482/33321 https://dx.doi.org/10.1590/0037-8682-0010-2018
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spelling	ir-10482-333212019-06-06T14:02:45Z The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome Alves, Daniella de Sousa Mendes Moreira Gonçalves, Gustavo Souza Moraes, Aline Silva Alves, Luciano Moreira Carmo Neto, José Rodrigues do Hecht, Mariana Machado Nitz, Nadjar Gonçalves, Rodrigo Gurgel Bernardes, Geisa Castro, Ana Maria de Chalita, Maria Regina Vinaud, Marina Clare Córnea - doenças Córnea - exames We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil. 2019-01-02T13:50:27Z 2019-01-02T13:50:27Z 2018 Artigo ALVES, Daniella de Sousa Mendes Moreira et al. The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 5, p. 716-719, set./out. 2018. DOI: http://dx.doi.org/10.1590/0037-8682-0010-2018. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500716&lng=en&nrm=iso. Acesso em: 06 jun. 2019. http://repositorio.unb.br/handle/10482/33321 https://dx.doi.org/10.1590/0037-8682-0010-2018 en Acesso Aberto Revista da Sociedade Brasileira de Medicina Tropical – (CC BY) - This is an open-access article distributed under the terms of the Creative Commons Attribution License. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500716&lng=en&tlng=en. Acesso em: 06 jun. 2019. application/pdf Sociedade Brasileira de Medicina Tropical - SBMT
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Córnea - doenças Córnea - exames
spellingShingle	Córnea - doenças Córnea - exames Alves, Daniella de Sousa Mendes Moreira Gonçalves, Gustavo Souza Moraes, Aline Silva Alves, Luciano Moreira Carmo Neto, José Rodrigues do Hecht, Mariana Machado Nitz, Nadjar Gonçalves, Rodrigo Gurgel Bernardes, Geisa Castro, Ana Maria de Chalita, Maria Regina Vinaud, Marina Clare The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
description	We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil.
format	Artigo
author	Alves, Daniella de Sousa Mendes Moreira Gonçalves, Gustavo Souza Moraes, Aline Silva Alves, Luciano Moreira Carmo Neto, José Rodrigues do Hecht, Mariana Machado Nitz, Nadjar Gonçalves, Rodrigo Gurgel Bernardes, Geisa Castro, Ana Maria de Chalita, Maria Regina Vinaud, Marina Clare
author_sort	Alves, Daniella de Sousa Mendes Moreira
title	The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
title_short	The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
title_full	The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
title_fullStr	The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
title_full_unstemmed	The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
title_sort	first acanthamoeba keratitis case in the midwest region of brazil: diagnosis, genotyping of the parasite and disease outcome
publisher	Sociedade Brasileira de Medicina Tropical - SBMT
publishDate	2019
url	http://repositorio.unb.br/handle/10482/33321 https://dx.doi.org/10.1590/0037-8682-0010-2018
_version_	1641989021679747072
score	13.657419

The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

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