Staff View: Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks

Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks

This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent....

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Main Authors:	Dantas, Alisson F., Lopes, Renata M., Fascineli, Maria Luiza, José, Solange C. B. R., Pádua, Juliano G., Gimenes, Marcos A., Grisolia, Cesar Koppe
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Genética 2019
Subjects:	Arroz Feijão Metanossulfonato de metila Citogenética
Online Access:	http://repositorio.unb.br/handle/10482/33561 http://dx.doi.org/10.1590/1678-4685-gmb-2017-0020
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spelling	ir-10482-335612021-05-31T13:05:49Z Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks Dantas, Alisson F. Lopes, Renata M. Fascineli, Maria Luiza José, Solange C. B. R. Pádua, Juliano G. Gimenes, Marcos A. Grisolia, Cesar Koppe Arroz Feijão Metanossulfonato de metila Citogenética This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used. 2019-01-02T13:53:53Z 2019-01-02T13:53:53Z 2018 Artigo DANTAS, Alisson F. et al. Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks. Genetics and Molecular Biology, Ribeirão Preto, v. 41, n. 1, p. 145-153, mar. 2018. DOI: http://dx.doi.org/10.1590/1678-4685-gmb-2017-0020. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100145&lng=en&nrm=iso. Acesso em: 11 mar. 2019. http://repositorio.unb.br/handle/10482/33561 http://dx.doi.org/10.1590/1678-4685-gmb-2017-0020 en Acesso Aberto License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. application/pdf Sociedade Brasileira de Genética
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Arroz Feijão Metanossulfonato de metila Citogenética
spellingShingle	Arroz Feijão Metanossulfonato de metila Citogenética Dantas, Alisson F. Lopes, Renata M. Fascineli, Maria Luiza José, Solange C. B. R. Pádua, Juliano G. Gimenes, Marcos A. Grisolia, Cesar Koppe Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
description	This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used.
format	Artigo
author	Dantas, Alisson F. Lopes, Renata M. Fascineli, Maria Luiza José, Solange C. B. R. Pádua, Juliano G. Gimenes, Marcos A. Grisolia, Cesar Koppe
author_sort	Dantas, Alisson F.
title	Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
title_short	Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
title_full	Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
title_fullStr	Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
title_full_unstemmed	Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
title_sort	comet and cytogenetic tests as tools for evaluating genomic instability in seeds of oryza sativa l. and phaseolus vulgaris l. from gene banks
publisher	Sociedade Brasileira de Genética
publishDate	2019
url	http://repositorio.unb.br/handle/10482/33561 http://dx.doi.org/10.1590/1678-4685-gmb-2017-0020
_version_	1710449493129822208
score	13.657419

Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks

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