Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity
Following the treads of our previous works on the unveiling of bioactive peptides encrypted in plant proteins from diverse species, the present manuscript reports the occurrence of four proof-of-concept intragenic antimicrobial peptides in human proteins, named Hs IAPs. These IAPs were prospected...
| Main Authors: | Brand, Guilherme Dotto, Ramada, Marcelo H. S., Manickchand, Júlia R., Correa, Rafael, Ribeiro, Dalila J. S., Santos, Michele A., Vasconcelos, Andreanne G., Abrão, Fernando Y., Prates, Maura V., Murad, André M., Cardozo Fh, José L., Leite, José Roberto S. A., Magalhães, Kelly G., Oliveira, Aline Lima de, Bloch Júnior, Carlos |
|---|---|
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Plos One
2019
|
| Subjects: | |
| Online Access: |
https://repositorio.unb.br/handle/10482/35803 https://orcid.org/0000-0002-1615-0009 https://orcid.org/0000-0002-6928-078X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
ir-10482-35803 |
|---|---|
| recordtype |
dspace |
| spelling |
ir-10482-358032019-11-20T20:38:20Z Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity Brand, Guilherme Dotto Ramada, Marcelo H. S. Manickchand, Júlia R. Correa, Rafael Ribeiro, Dalila J. S. Santos, Michele A. Vasconcelos, Andreanne G. Abrão, Fernando Y. Prates, Maura V. Murad, André M. Cardozo Fh, José L. Leite, José Roberto S. A. Magalhães, Kelly G. Oliveira, Aline Lima de Bloch Júnior, Carlos Peptídeos bioativos Agentes antiinflamatórios Agentes antiinfecciosos Following the treads of our previous works on the unveiling of bioactive peptides encrypted in plant proteins from diverse species, the present manuscript reports the occurrence of four proof-of-concept intragenic antimicrobial peptides in human proteins, named Hs IAPs. These IAPs were prospected using the software Kamal, synthesized by solid phase chemistry, and had their interactions with model phospholipid vesicles investigated by differential scanning calorimetry and circular dichroism. Their antimicrobial activity against bacteria, yeasts and filamentous fungi was determined, along with their cytotoxicity towards erythrocytes. Our data demonstrates that Hs IAPs are capable to bind model membranes while attaining α-helical structure, and to inhibit the growth of microorganisms at concentrations as low as 1μM. Hs02, a novel sixteen residue long internal peptide (KWAVRIIRKFIKGFISNH2) derived from the unconventional myosin 1h protein, was further investigated in its capacity to inhibit lipopolysaccharide-induced release of TNF-α in murine macrophages. Hs02 presented potent anti-inflammatory activity, inhibiting the release of TNF-α in LPSprimed cells at the lowest assayed concentration, 0.1 μM. A three-dimensional solution structure of Hs02 bound to DPC micelles was determined by Nuclear Magnetic Resonance. Our work exemplifies how the human genome can be mined for molecules with biotechnological potential in human health and demonstrates that IAPs are actual alternatives to antimicrobial peptides as pharmaceutical agents or in their many other putative applications. 2019-11-11T14:41:10Z 2019-11-11T14:41:10Z 2019 Artigo BRAND, GUILHERME D. et al. Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity. PLoS ONE, v. 14, n. 8, e0220656, 2019. DOI: https://doi.org/10.1371/journal.pone.0220656. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220656. Acesso em: 11 nov. 2019. https://repositorio.unb.br/handle/10482/35803 https://orcid.org/0000-0002-1615-0009 https://orcid.org/0000-0002-6928-078X Inglês Acesso Aberto © 2019 Brand et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf Plos One |
| institution |
REPOSITORIO UNB |
| collection |
REPOSITORIO UNB |
| language |
Inglês |
| topic |
Peptídeos bioativos Agentes antiinflamatórios Agentes antiinfecciosos |
| spellingShingle |
Peptídeos bioativos Agentes antiinflamatórios Agentes antiinfecciosos Brand, Guilherme Dotto Ramada, Marcelo H. S. Manickchand, Júlia R. Correa, Rafael Ribeiro, Dalila J. S. Santos, Michele A. Vasconcelos, Andreanne G. Abrão, Fernando Y. Prates, Maura V. Murad, André M. Cardozo Fh, José L. Leite, José Roberto S. A. Magalhães, Kelly G. Oliveira, Aline Lima de Bloch Júnior, Carlos Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| description |
Following the treads of our previous works on the unveiling of bioactive peptides encrypted
in plant proteins from diverse species, the present manuscript reports the occurrence of four
proof-of-concept intragenic antimicrobial peptides in human proteins, named Hs IAPs.
These IAPs were prospected using the software Kamal, synthesized by solid phase chemistry,
and had their interactions with model phospholipid vesicles investigated by differential
scanning calorimetry and circular dichroism. Their antimicrobial activity against bacteria,
yeasts and filamentous fungi was determined, along with their cytotoxicity towards erythrocytes.
Our data demonstrates that Hs IAPs are capable to bind model membranes while
attaining α-helical structure, and to inhibit the growth of microorganisms at concentrations
as low as 1μM. Hs02, a novel sixteen residue long internal peptide (KWAVRIIRKFIKGFISNH2)
derived from the unconventional myosin 1h protein, was further investigated in its
capacity to inhibit lipopolysaccharide-induced release of TNF-α in murine macrophages.
Hs02 presented potent anti-inflammatory activity, inhibiting the release of TNF-α in LPSprimed
cells at the lowest assayed concentration, 0.1 μM. A three-dimensional solution
structure of Hs02 bound to DPC micelles was determined by Nuclear Magnetic Resonance.
Our work exemplifies how the human genome can be mined for molecules with biotechnological
potential in human health and demonstrates that IAPs are actual alternatives to antimicrobial
peptides as pharmaceutical agents or in their many other putative applications. |
| format |
Artigo |
| author |
Brand, Guilherme Dotto Ramada, Marcelo H. S. Manickchand, Júlia R. Correa, Rafael Ribeiro, Dalila J. S. Santos, Michele A. Vasconcelos, Andreanne G. Abrão, Fernando Y. Prates, Maura V. Murad, André M. Cardozo Fh, José L. Leite, José Roberto S. A. Magalhães, Kelly G. Oliveira, Aline Lima de Bloch Júnior, Carlos |
| author_sort |
Brand, Guilherme Dotto |
| title |
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| title_short |
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| title_full |
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| title_fullStr |
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| title_full_unstemmed |
Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity |
| title_sort |
intragenic antimicrobial peptides (iaps) from human proteins with potent antimicrobial and anti-inflammatory activity |
| publisher |
Plos One |
| publishDate |
2019 |
| url |
https://repositorio.unb.br/handle/10482/35803 https://orcid.org/0000-0002-1615-0009 https://orcid.org/0000-0002-6928-078X |
| _version_ |
1672205501280026624 |
| score |
13.657419 |