Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum

Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate...

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Main Authors: Costa, Gabriella Gusmão Pereira da, Silva, Cristian Aldemar Gasca, Gomes, João Victor Dutra, Torres, Amanda Gomes, Santos, Izulmé Rita Imaculada, Almeida, Fabrício Tavares Cunha de, Fagg, Christopher William, Simeoni, Luiz Alberto, Silveira, Dâmaris, Gomes-Copeland, Kicia Karinne Pereira
Format: Artigo
Language: English
Published: Sociedade Brasileira de Farmacognosia 2020
Subjects:
Online Access: https://repositorio.unb.br/handle/10482/36338
https://doi.org/10.1016/j.bjp.2019.02.001
http://orcid.org/0000-0002-3393-5609
http://orcid.org/0000-0003-4392-4230
http://orcid.org/0000-0001-6098-3852
http://orcid.org/0000-0001-9599-4578
http://orcid.org/0000-0002-1503-1497
http://orcid.org/0000-0002-0531-7483
http://orcid.org/0000-0001-6072-7832
http://orcid.org/0000-0003-3401-290X
http://orcid.org/0000-0003-1851-5224
http://orcid.org/0000-0003-3539-2916
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Summary: Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity.