Staff View: Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy

Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy

Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast...

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Main Authors:	Maria, Roberta M., Altei, Wanessa F., Valadares, Napoleão Fonseca, Garratt, Richard C., Andricopulo, Adriano D., Venâncio, Tiago, Colnago, Luiz A.
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Química 2020
Subjects:	Ácido linoleico Metabolômica Mamas - câncer Ressonância magnética nuclear
Online Access:	https://repositorio.unb.br/handle/10482/36369 https://doi.org/10.21577/0103-5053.20180200 http://orcid.org/0000-0002-2390-1843
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spelling	ir-10482-363692020-08-21T18:02:59Z Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy Maria, Roberta M. Altei, Wanessa F. Valadares, Napoleão Fonseca Garratt, Richard C. Andricopulo, Adriano D. Venâncio, Tiago Colnago, Luiz A. Ácido linoleico Metabolômica Mamas - câncer Ressonância magnética nuclear Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast cancer cells using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. The NMR spectra showed that phosphocholine level, a cancer malignance biomarker, was reduced in both cells treated with CLA, but the reduction was more pronounced in MCF-7 cells. The NMR spectra also showed that CLA has opposite effect on MCF-7 and MDA-MB-231 acetone metabolism. Acetone signal has been observed in the spectra of MDA-MB-231 control cells, but not in the spectra of the cells treated with 50 and 100 µM CLA. Conversely, the acetone signal is very small or not observed in the NMR spectra of MCF-7 control cells and in cells treated with 50 µM of CLA, but is very strong in the spectra of the cells treated with 100 µM of CLA. Therefore, this CLA concentration is causing a ketosis in MCF-7 cells by inducing the use of fatty acids as an energy source or by reducing acetone catabolism. These results indicate that CLA interfere in the metabolism of both cells. However, the strongest effect has been observed on the metabolism of MCF-7 cells cultivated in the presence of 100 µM CLA. Therefore, CLA could be a potential anticarcinogenic drug, especially for cells with positive estrogen receptor, such as MCF-7. 2020-01-24T10:30:30Z 2020-01-24T10:30:30Z 2019-01 Artigo MARIA, Roberta M. et al. Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 3-7, 2019. DOI: https://doi.org/10.21577/0103-5053.20180200. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000100003. Acesso em: 23 jan. 2020. https://repositorio.unb.br/handle/10482/36369 https://doi.org/10.21577/0103-5053.20180200 http://orcid.org/0000-0002-2390-1843 en Acesso Aberto (CC BY) - This is an open-access article distributed under the terms of the Creative Commons Attribution License. application/pdf Sociedade Brasileira de Química
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Ácido linoleico Metabolômica Mamas - câncer Ressonância magnética nuclear
spellingShingle	Ácido linoleico Metabolômica Mamas - câncer Ressonância magnética nuclear Maria, Roberta M. Altei, Wanessa F. Valadares, Napoleão Fonseca Garratt, Richard C. Andricopulo, Adriano D. Venâncio, Tiago Colnago, Luiz A. Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
description	Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast cancer cells using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. The NMR spectra showed that phosphocholine level, a cancer malignance biomarker, was reduced in both cells treated with CLA, but the reduction was more pronounced in MCF-7 cells. The NMR spectra also showed that CLA has opposite effect on MCF-7 and MDA-MB-231 acetone metabolism. Acetone signal has been observed in the spectra of MDA-MB-231 control cells, but not in the spectra of the cells treated with 50 and 100 µM CLA. Conversely, the acetone signal is very small or not observed in the NMR spectra of MCF-7 control cells and in cells treated with 50 µM of CLA, but is very strong in the spectra of the cells treated with 100 µM of CLA. Therefore, this CLA concentration is causing a ketosis in MCF-7 cells by inducing the use of fatty acids as an energy source or by reducing acetone catabolism. These results indicate that CLA interfere in the metabolism of both cells. However, the strongest effect has been observed on the metabolism of MCF-7 cells cultivated in the presence of 100 µM CLA. Therefore, CLA could be a potential anticarcinogenic drug, especially for cells with positive estrogen receptor, such as MCF-7.
format	Artigo
author	Maria, Roberta M. Altei, Wanessa F. Valadares, Napoleão Fonseca Garratt, Richard C. Andricopulo, Adriano D. Venâncio, Tiago Colnago, Luiz A.
author_sort	Maria, Roberta M.
title	Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
title_short	Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
title_full	Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
title_fullStr	Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
title_full_unstemmed	Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy
title_sort	effect of cis-9, trans-11 conjugated linoleic acid (cla) on the metabolism profile of breast cancer cells determined by h hr-mas nmr spectroscopy
publisher	Sociedade Brasileira de Química
publishDate	2020
url	https://repositorio.unb.br/handle/10482/36369 https://doi.org/10.21577/0103-5053.20180200 http://orcid.org/0000-0002-2390-1843
_version_	1680624761480151040
score	13.657419

Effect of cis-9, trans-11 conjugated linoleic acid (CLA) on the metabolism profile of breast cancer cells determined by H HR-MAS NMR spectroscopy

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