Staff View: Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs

Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs

Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-produ...

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Main Authors:	Paim, Tiago do Prado, Viana, Pauline Galvão, Tilburg, Mauricio Fraga van, Moura, Arlindo de Alencar, Souza, Jurandir Rodrigues de, Pimentel, Concepta Margaret McManus, Abdalla, Adibe Luiz, Louvandini, Helder
Format:	Artigo
Language:	English
Published:	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" 2020
Subjects:	Gossipol livre Desidrogenase Sistema musculoesquelético Ovelha Confinamento (Animais)
Online Access:	https://repositorio.unb.br/handle/10482/36384 https://doi.org/10.1590/1678-992x-2018-0072 http://orcid.org/0000-0002-9486-7128 http://orcid.org/0000-0003-2157-3986 http://orcid.org/0000-0001-5819-0861 http://orcid.org/0000-0002-8271-5733 http://orcid.org/0000-0003-1646-4304 http://orcid.org/0000-0002-1106-8962 http://orcid.org/0000-0002-5440-9974 http://orcid.org/0000-0001-7129-8463
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spelling	ir-10482-363842020-07-02T12:50:41Z Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs Paim, Tiago do Prado Viana, Pauline Galvão Tilburg, Mauricio Fraga van Moura, Arlindo de Alencar Souza, Jurandir Rodrigues de Pimentel, Concepta Margaret McManus Abdalla, Adibe Luiz Louvandini, Helder Gossipol livre Desidrogenase Sistema musculoesquelético Ovelha Confinamento (Animais) Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations. 2020-01-24T10:30:35Z 2020-01-24T10:30:35Z 2019 Artigo PAIM, Tiago do Prado et al. Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs. Scientia Agricola, v. 76, n. 6, p. 463-472, nov./dez. 2019. DOI: https://doi.org/10.1590/1678-992x-2018-0072. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600463. Acesso em: 23 jan. 2020. https://repositorio.unb.br/handle/10482/36384 https://doi.org/10.1590/1678-992x-2018-0072 http://orcid.org/0000-0002-9486-7128 http://orcid.org/0000-0003-2157-3986 http://orcid.org/0000-0001-5819-0861 http://orcid.org/0000-0002-8271-5733 http://orcid.org/0000-0003-1646-4304 http://orcid.org/0000-0002-1106-8962 http://orcid.org/0000-0002-5440-9974 http://orcid.org/0000-0001-7129-8463 en Acesso Aberto (CC BY) application/pdf São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Gossipol livre Desidrogenase Sistema musculoesquelético Ovelha Confinamento (Animais)
spellingShingle	Gossipol livre Desidrogenase Sistema musculoesquelético Ovelha Confinamento (Animais) Paim, Tiago do Prado Viana, Pauline Galvão Tilburg, Mauricio Fraga van Moura, Arlindo de Alencar Souza, Jurandir Rodrigues de Pimentel, Concepta Margaret McManus Abdalla, Adibe Luiz Louvandini, Helder Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
description	Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.
format	Artigo
author	Paim, Tiago do Prado Viana, Pauline Galvão Tilburg, Mauricio Fraga van Moura, Arlindo de Alencar Souza, Jurandir Rodrigues de Pimentel, Concepta Margaret McManus Abdalla, Adibe Luiz Louvandini, Helder
author_sort	Paim, Tiago do Prado
title	Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_short	Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_full	Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_fullStr	Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_full_unstemmed	Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
title_sort	feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
publisher	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
publishDate	2020
url	https://repositorio.unb.br/handle/10482/36384 https://doi.org/10.1590/1678-992x-2018-0072 http://orcid.org/0000-0002-9486-7128 http://orcid.org/0000-0003-2157-3986 http://orcid.org/0000-0001-5819-0861 http://orcid.org/0000-0002-8271-5733 http://orcid.org/0000-0003-1646-4304 http://orcid.org/0000-0002-1106-8962 http://orcid.org/0000-0002-5440-9974 http://orcid.org/0000-0001-7129-8463
_version_	1672205855538282496
score	13.657419

Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs

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