Staff View: In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)

In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)

The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is...

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Main Authors:	Lima-Rezende, Cássia Alves, Fernandes, Gislaine Aparecida, Silva, Helder Elias da, Dobkowski-Marinho, Sarah, Santos, Victor Fernandes, Rodrigues, Fernando Pacheco, Caparroz, Renato
Format:	Artigo
Language:	English
Published:	Sociedade Brasileira de Genética 2020
Subjects:	Animais selvagens em cativeiro Tráfico de animais Parentesco Papagaio (Ave) Vida selvagem - conservação
Online Access:	https://repositorio.unb.br/handle/10482/36475 https://doi.org/10.1590/1678-4685-gmb-2017-0338 http://orcid.org/0000-0003-4883-4023
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spelling	ir-10482-364752020-09-18T16:41:02Z In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae) Lima-Rezende, Cássia Alves Fernandes, Gislaine Aparecida Silva, Helder Elias da Dobkowski-Marinho, Sarah Santos, Victor Fernandes Rodrigues, Fernando Pacheco Caparroz, Renato Animais selvagens em cativeiro Tráfico de animais Parentesco Papagaio (Ave) Vida selvagem - conservação The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is under protection from over-exploitation. This study aimed to identify and characterize novel microsatellite loci for population and parentage analysis of A. ararauna. Scaffold sequences of Ara macao available in the NCBI database were used for microsatellite searches using MsatCommander software. We tested a total of 28 loci, from which 25 were polymorphic, one was monomorphic, and two did not generated amplification products. For polymorphic loci, the mean number of alleles was 8.24 (4 – 15 alleles per locus), the observed heterozygosity ranged from 0.333 to 0.917, and the expected heterozygosity from 0.353 to 0.890. The paternity exclusion probability and identity probability were highly discriminatory. Thus, these novel microsatellite markers can be useful for population assignment and paternity tests, helping the authorities to manage macaws from the illegal trafficking and control commercial breeders. 2020-01-24T10:31:09Z 2020-01-24T10:31:09Z 2019 Artigo LIMA-REZENDE, Cássia Alves et al. In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae). Genetics and Molecular Biology, v. 42, n. 1, p. 68-73, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2017-0338. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100068. Acesso em: 23 jan. 2020. https://repositorio.unb.br/handle/10482/36475 https://doi.org/10.1590/1678-4685-gmb-2017-0338 http://orcid.org/0000-0003-4883-4023 en Acesso Aberto License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. application/pdf Sociedade Brasileira de Genética
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Animais selvagens em cativeiro Tráfico de animais Parentesco Papagaio (Ave) Vida selvagem - conservação
spellingShingle	Animais selvagens em cativeiro Tráfico de animais Parentesco Papagaio (Ave) Vida selvagem - conservação Lima-Rezende, Cássia Alves Fernandes, Gislaine Aparecida Silva, Helder Elias da Dobkowski-Marinho, Sarah Santos, Victor Fernandes Rodrigues, Fernando Pacheco Caparroz, Renato In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
description	The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is under protection from over-exploitation. This study aimed to identify and characterize novel microsatellite loci for population and parentage analysis of A. ararauna. Scaffold sequences of Ara macao available in the NCBI database were used for microsatellite searches using MsatCommander software. We tested a total of 28 loci, from which 25 were polymorphic, one was monomorphic, and two did not generated amplification products. For polymorphic loci, the mean number of alleles was 8.24 (4 – 15 alleles per locus), the observed heterozygosity ranged from 0.333 to 0.917, and the expected heterozygosity from 0.353 to 0.890. The paternity exclusion probability and identity probability were highly discriminatory. Thus, these novel microsatellite markers can be useful for population assignment and paternity tests, helping the authorities to manage macaws from the illegal trafficking and control commercial breeders.
format	Artigo
author	Lima-Rezende, Cássia Alves Fernandes, Gislaine Aparecida Silva, Helder Elias da Dobkowski-Marinho, Sarah Santos, Victor Fernandes Rodrigues, Fernando Pacheco Caparroz, Renato
author_sort	Lima-Rezende, Cássia Alves
title	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
title_short	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
title_full	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
title_fullStr	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
title_full_unstemmed	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
title_sort	in silico identification and characterization of novel microsatellite loci for the blue-and-yellow macaw ara ararauna (linnaeus, 1758) (psittaciformes, psittacidae)
publisher	Sociedade Brasileira de Genética
publishDate	2020
url	https://repositorio.unb.br/handle/10482/36475 https://doi.org/10.1590/1678-4685-gmb-2017-0338 http://orcid.org/0000-0003-4883-4023
_version_	1680624725235073024
score	13.657419

In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)

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