Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential
Ayahuasca is a psychoactive infusion with a large pharmacological application normally prepared with Banisteriopsis caapi, which contains the monoamine oxidase inhibitors β-carbolines, and Psichotria virids, which contains the serotonin receptor agonist N,N dimethyltryptamine (DMT). The objective...
| Main Authors: | Santos, Beatriz Werneck Lopes, Oliveira, Regina Célia de, Oliveira, Julia Sonsin, Fagg, Christopher William, Barbosa, José Beethoven Figueiredo, Caldas, Eloisa Dutra |
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| Language: | Inglês |
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2020
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https://repositorio.unb.br/handle/10482/39296 https://doi.org/10.3390/plants9070870 https://orcid.org/0000-0003-0486-3690 https://orcid.org/0000-0001-7891-2630 https://orcid.org/0000-0002-4905-8145 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0002-7197-6807 |
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ir-10482-392962020-07-24T19:52:14Z Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential Santos, Beatriz Werneck Lopes Oliveira, Regina Célia de Oliveira, Julia Sonsin Fagg, Christopher William Barbosa, José Beethoven Figueiredo Caldas, Eloisa Dutra Betacarbolina Cipó-mariri Ayahuasca Ayahuasca is a psychoactive infusion with a large pharmacological application normally prepared with Banisteriopsis caapi, which contains the monoamine oxidase inhibitors β-carbolines, and Psichotria virids, which contains the serotonin receptor agonist N,N dimethyltryptamine (DMT). The objectives of this study were to investigate the chemical profile of B. caapi and of ayahuasca collected in various Brazilian regions. In total, 176 plant lianas, of which 159 B. caapi and 33 ayahuasca samples were analyzed. Dried liana samples were powdered, extracted with methanol, diluted, and analyzed by LC-MS/MS. Ayahuasca samples were diluted and analyzed. Mean concentrations in B. caapi were 4.79 mg/g harmine, 0.451 mg/g harmaline, and 2.18 mg/g tetrahydroharmine (THH), with a high variability among the samples (RSD from 78.9 to 170%). Native B. caapi samples showed significantly higher harmine concentrations than cultivated ones, and samples from the Federal District/Goiás had higher THH content than those collected in the State of Acre. The other Malpighiaceae samples did not contain β-carbolines, except for one D. pubipetala sample. Concentrations in ayahuasca samples ranged from 0.109 to 7.11 mg/mL harmine, 0.012 to 0.945 mg/mL harmaline, 0.09 to 3.05 mg/mL THH, and 0.10 to 3.12 mg/mL DMT. The analysis of paired ayahuasca/B. caapi confirmed that harmine is reduced to harmaline and to THH during the brew preparation. This is the largest study conducted with Malpighiaceae samples and showed a large variability in the main β-carbolines present in B. caapi. This biodiversity is a challenge for standardization of the material used in ethnopharmacological studies of B. caapi and ayahuasca. 2020-07-24T19:42:33Z 2020-07-24T19:42:33Z 2020-07-09 Artigo SANTOS, Beatriz Werneck Lopes et al. Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential. Plants, v. 9, n. 7, 870. DOI: https://doi.org/10.3390/plants9070870. Disponível em: https://www.mdpi.com/2223-7747/9/7/870. Acesso em: 24 jul. 2020. https://repositorio.unb.br/handle/10482/39296 https://doi.org/10.3390/plants9070870 https://orcid.org/0000-0003-0486-3690 https://orcid.org/0000-0001-7891-2630 https://orcid.org/0000-0002-4905-8145 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0002-7197-6807 Inglês Acesso Aberto © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf MDPI |
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| language |
Inglês |
| topic |
Betacarbolina Cipó-mariri Ayahuasca |
| spellingShingle |
Betacarbolina Cipó-mariri Ayahuasca Santos, Beatriz Werneck Lopes Oliveira, Regina Célia de Oliveira, Julia Sonsin Fagg, Christopher William Barbosa, José Beethoven Figueiredo Caldas, Eloisa Dutra Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| description |
Ayahuasca is a psychoactive infusion with a large pharmacological application normally
prepared with Banisteriopsis caapi, which contains the monoamine oxidase inhibitors β-carbolines,
and Psichotria virids, which contains the serotonin receptor agonist N,N dimethyltryptamine (DMT).
The objectives of this study were to investigate the chemical profile of B. caapi and of ayahuasca collected
in various Brazilian regions. In total, 176 plant lianas, of which 159 B. caapi and 33 ayahuasca samples
were analyzed. Dried liana samples were powdered, extracted with methanol, diluted, and analyzed
by LC-MS/MS. Ayahuasca samples were diluted and analyzed. Mean concentrations in B. caapi were
4.79 mg/g harmine, 0.451 mg/g harmaline, and 2.18 mg/g tetrahydroharmine (THH), with a high
variability among the samples (RSD from 78.9 to 170%). Native B. caapi samples showed significantly
higher harmine concentrations than cultivated ones, and samples from the Federal District/Goiás had
higher THH content than those collected in the State of Acre. The other Malpighiaceae samples did
not contain β-carbolines, except for one D. pubipetala sample. Concentrations in ayahuasca samples
ranged from 0.109 to 7.11 mg/mL harmine, 0.012 to 0.945 mg/mL harmaline, 0.09 to 3.05 mg/mL THH,
and 0.10 to 3.12 mg/mL DMT. The analysis of paired ayahuasca/B. caapi confirmed that harmine is
reduced to harmaline and to THH during the brew preparation. This is the largest study conducted
with Malpighiaceae samples and showed a large variability in the main β-carbolines present in B. caapi.
This biodiversity is a challenge for standardization of the material used in ethnopharmacological
studies of B. caapi and ayahuasca. |
| format |
Artigo |
| author |
Santos, Beatriz Werneck Lopes Oliveira, Regina Célia de Oliveira, Julia Sonsin Fagg, Christopher William Barbosa, José Beethoven Figueiredo Caldas, Eloisa Dutra |
| author_sort |
Santos, Beatriz Werneck Lopes |
| title |
Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| title_short |
Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| title_full |
Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| title_fullStr |
Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| title_full_unstemmed |
Biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| title_sort |
biodiversity of β-carboline profile of banisteriopsis caapi and ayahuasca, a plant and a brew with neuropharmacological potential |
| publisher |
MDPI |
| publishDate |
2020 |
| url |
https://repositorio.unb.br/handle/10482/39296 https://doi.org/10.3390/plants9070870 https://orcid.org/0000-0003-0486-3690 https://orcid.org/0000-0001-7891-2630 https://orcid.org/0000-0002-4905-8145 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0002-7197-6807 |
| _version_ |
1675464131360063488 |
| score |
13.657419 |