Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract
We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were...
| Main Authors: | Tormena, Renata Pascoal Illanes, Rosa, Eliane Vieira, Mota, Bruna de Fatima Oliveira, Chaker, Juliano Alexandre, Fagg, Christopher William, Freire, Daniel Oliveira, Martins, Paula Melo, Silva, Izabel Cristina Rodrigues da, Sousa, Marcelo Henrique |
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| Language: | Inglês |
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The Royal Society of Chemistry
2020
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https://repositorio.unb.br/handle/10482/39621 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
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ir-10482-396212020-11-10T17:36:45Z Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract Tormena, Renata Pascoal Illanes Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique Atividade antimicrobiana Nanopartículas de prata Ipê-roxo We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. 2020-11-10T17:32:09Z 2020-11-10T17:32:09Z 2020-06-01 Artigo TORMENA, Renata Pascoal Illanes et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, v. 10, 20676-20681, 2020. DOI: https://doi.org/10.1039/D0RA03240A. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A#!divAbstract. Acesso em: 10 nov. 2020. https://repositorio.unb.br/handle/10482/39621 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 Inglês Acesso Aberto Open Access Article - (CC BY-NC) - This article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence. application/pdf The Royal Society of Chemistry |
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Inglês |
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Atividade antimicrobiana Nanopartículas de prata Ipê-roxo |
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Atividade antimicrobiana Nanopartículas de prata Ipê-roxo Tormena, Renata Pascoal Illanes Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| description |
We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. |
| format |
Artigo |
| author |
Tormena, Renata Pascoal Illanes Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
| author_sort |
Tormena, Renata Pascoal Illanes |
| title |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_short |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_full |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_fullStr |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_full_unstemmed |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_sort |
evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using handroanthus impetiginosus (mart. ex dc.) mattos underbark extract |
| publisher |
The Royal Society of Chemistry |
| publishDate |
2020 |
| url |
https://repositorio.unb.br/handle/10482/39621 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
| _version_ |
1683063395797958656 |
| score |
13.657419 |