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Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax

ABSTRACT: The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500...

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Main Authors: Obando, Hernando Antonio del Portillo, Levitus, Gabriela, Camargo, Luiz Marcelo Aranha, Ferreira, Marcelo Urbano, Mertens, Frédéric
Format: Artigo
Language: Inglês
Published: 2010
Subjects:
Protozoário
Amazônia
Malária
Online Access: http://repositorio.unb.br/handle/10482/5851
https://dx.doi.org/10.1590/S0074-02761992000700010
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spelling ir-10482-58512020-04-24T17:06:48Z Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax Obando, Hernando Antonio del Portillo Levitus, Gabriela Camargo, Luiz Marcelo Aranha Ferreira, Marcelo Urbano Mertens, Frédéric Protozoário Amazônia Malária ABSTRACT: The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. _______________________________________________________________________________ RESUMO A estrutura primária completa do gene que codifica a proteína de superfície Merozoite 1 de Plasmodium vivax (PvMSP-1) revelou a existência de regiões conservadas entre espécies entre os análogos de proteínas de outras espécies de plasmódios. Aqui, três clones de DNA recombinante expressando 50, 200 e 500 aminoácidos da região N-terminal da proteína-1 PvMSP foram utilizados em ensaios de ELISA e immunoblotting proteína de olhar para as respostas de anticorpos IgG de pacientes com malária da Amazônia Brasileira de Rondônia. Os resultados mostraram a existência de P. vivax e P. falciparum anticorpos IgG dirigidos contra PvMSP-1 determinantes antigênicos expressos nos clones contendo os primeiros 200 e os 500 seguintes aminoácidos da molécula, mas não dentro da uma expressão mais N- terminal de 50 aminoácidos. Curiosamente, não houve correlação entre os níveis destes anticorpos IgG e no número anterior de malária. 2010-11-09T12:58:37Z 2010-11-09T12:58:37Z 1992 Artigo OBANDO, Hernando Antonio del Portillo et al. Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax. Memórias do Instituto Oswaldo Cruz, v. 87, sup. 3, p. 77-84, 1992. Disponível em: <http://www.scielo.br/pdf/mioc/v87s3/vol87%28fsup3%29_083-090.pdf>. Acesso em: 5 nov. 2010. doi: 10.1590/S0074-02761992000700010. http://repositorio.unb.br/handle/10482/5851 https://dx.doi.org/10.1590/S0074-02761992000700010 Inglês Acesso Aberto application/pdf
institution REPOSITORIO UNB
collection REPOSITORIO UNB
language Inglês
topic Protozoário
Amazônia
Malária
spellingShingle Protozoário
Amazônia
Malária
Obando, Hernando Antonio del Portillo
Levitus, Gabriela
Camargo, Luiz Marcelo Aranha
Ferreira, Marcelo Urbano
Mertens, Frédéric
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
description ABSTRACT: The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. _______________________________________________________________________________ RESUMO
format Artigo
author Obando, Hernando Antonio del Portillo
Levitus, Gabriela
Camargo, Luiz Marcelo Aranha
Ferreira, Marcelo Urbano
Mertens, Frédéric
author_sort Obando, Hernando Antonio del Portillo
title Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_short Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_full Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_fullStr Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_full_unstemmed Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_sort human igg responses against the n-terminal region of merozoite surface protein 1 of plasmodium vivax
publishDate 2010
url http://repositorio.unb.br/handle/10482/5851
https://dx.doi.org/10.1590/S0074-02761992000700010
_version_ 1672205663736954880
score 13.657419

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