RT-PCR and Dot Blot hybridization methods for a universal detection of tospoviruses

Transcriptase reverse - polymerase chain reaction (RT-PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysa...

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Main Authors: EIRAS, MARCELO, RESENDE, RENATO O., MISSIAGGIA, ALEXANDRE A., ÁVILA, ANTÔNIO C. DE
Format: Artigo
Language: English
Published: Sociedade Brasileira de Fitopatologia 2017
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Online Access: http://repositorio.unb.br/handle/10482/25723
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