Staff View: Detection of three Allexivirus species infecting garlic in Brazil

Detection of three Allexivirus species infecting garlic in Brazil

Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (Gar...

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Main Authors:	Melo Filho, Péricles de Albuquerque, Nagata, Tatsuya, Dusi, André Nepomuceno, Buso, José Amauri, Torres, Antonio Carlos, Eiras, Marcelo, Resende, Renato de Oliveira
Format:	Artigo
Language:	English
Published:	Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira 2017
Subjects:	Allium sativum virus detection coat protein Dot-ELISA RT-PCR detecção de vírus proteínas capsidiais
Online Access:	http://repositorio.unb.br/handle/10482/26161
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spelling	ir-10482-261612019-03-28T14:05:16Z Detection of three Allexivirus species infecting garlic in Brazil Detecção de três espécies de Allexivirus que infectam o alho no Brasil Melo Filho, Péricles de Albuquerque Nagata, Tatsuya Dusi, André Nepomuceno Buso, José Amauri Torres, Antonio Carlos Eiras, Marcelo Resende, Renato de Oliveira Allium sativum virus detection coat protein Dot-ELISA RT-PCR Allium sativum detecção de vírus proteínas capsidiais Dot-ELISA RT-PCR Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil. Infecções virais em alho são normalmente causadas por um complexo viral. Neste estudo, um complexo viral de alho, coletado em campo, em três regiões geográficas, foi testado com anti-soros monoclonais específicos para Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) e um anti-soro policlonal capaz de detectar os três vírus mencionados e Garlic virus D (GarV-D). Procedeu-se à amplificação por transcriptase reversa-reação em cadeia da polimerase (RT-PCR) usando oligonucleotídeos sintetizados a partir de regiões específicas de genes de proteínas capsidiais de allexivirus japoneses e disponíveis no GeneBank (National Center of Biotechnology Information - NCBI). Por esse procedimento, vírus individuais foram isolados e seqüenciados. Os vírus detectados foram biologicamente isolados por meio de sucessivas inoculações em Chenopodium quinoa. A análise das seqüências de nucleotídeos, de aminoácidos e os resultados sorológicos revelaram a presença de três espécies distintas de allexivirus GarV-C, GarV-D e Garlic mite-borne filamentous virus (GarMbFV) no Brasil. 2017-12-07T04:39:14Z 2017-12-07T04:39:14Z 2004 Artigo Pesq. agropec. bras.,v.39,n.8,p.735-740,2004 0100-204X http://repositorio.unb.br/handle/10482/26161 10.1590/S0100-204X2004000800002 en Acesso Aberto application/pdf Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800002&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-204X2004000800002&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-204X2004000800002&lng=en&nrm=iso
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Allium sativum virus detection coat protein Dot-ELISA RT-PCR Allium sativum detecção de vírus proteínas capsidiais Dot-ELISA RT-PCR
spellingShingle	Allium sativum virus detection coat protein Dot-ELISA RT-PCR Allium sativum detecção de vírus proteínas capsidiais Dot-ELISA RT-PCR Melo Filho, Péricles de Albuquerque Nagata, Tatsuya Dusi, André Nepomuceno Buso, José Amauri Torres, Antonio Carlos Eiras, Marcelo Resende, Renato de Oliveira Detection of three Allexivirus species infecting garlic in Brazil
description	Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.
format	Artigo
author	Melo Filho, Péricles de Albuquerque Nagata, Tatsuya Dusi, André Nepomuceno Buso, José Amauri Torres, Antonio Carlos Eiras, Marcelo Resende, Renato de Oliveira
author_sort	Melo Filho, Péricles de Albuquerque
title	Detection of three Allexivirus species infecting garlic in Brazil
title_short	Detection of three Allexivirus species infecting garlic in Brazil
title_full	Detection of three Allexivirus species infecting garlic in Brazil
title_fullStr	Detection of three Allexivirus species infecting garlic in Brazil
title_full_unstemmed	Detection of three Allexivirus species infecting garlic in Brazil
title_sort	detection of three allexivirus species infecting garlic in brazil
publisher	Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira
publishDate	2017
url	http://repositorio.unb.br/handle/10482/26161
_version_	1641988689722605568
score	13.657419

Detection of three Allexivirus species infecting garlic in Brazil

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