Staff View: Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal

Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal

Epizootiological study of Anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. To achieve diagnosis of this rickettsia in cattle and campeiro deer of Brazilian Pantanal, a comparison was made between a re...

Full description

Main Authors:	Picoloto, Graziela, Lima, Renileide Ferreira de, Olegário, Lílian Andressa Oliveira, Carvalho, Cristiano Miranda Espínola, Lacerda, Ana Crystina Reis, Tomás, Walfrido Moraes, Borges, Paulo André Lima, Pellegrin, Aiesca Oliveira, Madruga, Cláudio Roberto
Format:	Artigo
Language:	English
Published:	Colégio Brasileiro de Parasitologia Veterinária 2017
Subjects:	Anaplasma marginale real time PCR cattle campeiro deer Pantanal PCR tempo real bovino veado campeiro
Online Access:	http://repositorio.unb.br/handle/10482/27920
Tags:	Add Tag No Tags, Be the first to tag this record!

id	ir-10482-27920
recordtype	dspace
spelling	ir-10482-279202019-03-28T14:02:26Z Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal Reação da polimerase em cadeia tempo real para diagnóstico de Anaplasma marginale em bovino e veado campeiro do Pantanal brasileiro Ozotoceros bezoarticus leucogaster Picoloto, Graziela Lima, Renileide Ferreira de Olegário, Lílian Andressa Oliveira Carvalho, Cristiano Miranda Espínola Lacerda, Ana Crystina Reis Tomás, Walfrido Moraes Borges, Paulo André Lima Pellegrin, Aiesca Oliveira Madruga, Cláudio Roberto Anaplasma marginale real time PCR cattle campeiro deer Pantanal Anaplasma marginale PCR tempo real bovino veado campeiro Pantanal Epizootiological study of Anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. To achieve diagnosis of this rickettsia in cattle and campeiro deer of Brazilian Pantanal, a comparison was made between a real time polymerase chain reaction (RT-PCR) with intercalating Sybr Green fluorochrome and primers based on msp5 gene of A. marginale; a conventional PCR (C-PCR); and parasitological examination using thin blood smear stained with Giemsa-MayGrunwald. Both PCRs showed good performance in the diagnosis of A. marginale in cattle, and were superior to the parasitological exam. The RT-PCR detected seven positive campeiro deer (16.3%). This rate was significantly higher compared to C-PCR, which identified one animal as positive (2.3%), and also compared to parasitological diagnosis, which did not find any positive animals. The dissociation temperature average of positive reactions in cattle (81.72 ºC ± 0.20) was identical to dissociation temperature found in the cervids (81.72 ºC ± 0.12), suggesting that both animal species were infected with A. marginale. We concluded that RT-PCR can be used for A. marginale diagnosis and in epizootiological studies of cattle and cervids; in spite of the small number of campeiro deer samples, the results indicated that this wildlife species has importance in the Anaplasma epizootiology in the Brazilian Pantanal. O estudo epizootiológico de Anaplasma marginale em regiões que existem vários reservatórios, co-existência de espécies de riquétsias patógenas e vetores comuns é uma tarefa complicada. Com o objetivo de obter o diagnóstico dessa riquétsia em bovinos e veado campeiro do Pantanal brasileiro foi avaliada uma reação da polimerase em cadeia em tempo real (PCR-TR) com o fluoróforo intercalante de fita dupla de DNA Sybr Green e iniciadores baseados na seqüência do gene msp5 de A. marginale comparando-a a uma PCR convencional (PCR-C) e ao exame parasitológico de esfregaço fino de sangue corado com Giemsa-MayGrunwald. Ambas PCRs apresentaram bom desempenho no diagnóstico de A. marginale nos bovinos, o qual foi superior ao exame parasitológico. O PCR-TR detectou sete veados campeiros positivos (16,3%), o que foi significativamente maior comparado ao PCR-C identificando um animal como positivo (2,3%), e ao exame parasitológico não encontrou nenhum animal positivo. A média da temperatura de dissociação das reações positivas para amostras de bovinos (81,72 ºC ± 0,20) foi idêntica àquelas dos cervídeos ( 81,72 ºC ± 0,12), o que sugere que ambas espécies animais foram infectadas por A. marginale. Concluímos que PCR-TR pode ser utilizada para diagnóstico e estudos epizootiológicos de A. marginale em bovinos e cervídeos. Apesar da pequena amostragem de veado campeiro os resultados indicam que essa espécie de animal selvagem tem importância na epizootiologia do Anaplasma no Pantanal brasileiro. 2017-12-07T04:55:43Z 2017-12-07T04:55:43Z 2010 Artigo Rev. Bras. Parasitol. Vet. (Online),v.19,n.3,p.186-188,2010 1984-2961 http://repositorio.unb.br/handle/10482/27920 10.1590/S1984-29612010000300012 en Acesso Aberto application/pdf Colégio Brasileiro de Parasitologia Veterinária http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000300012&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1984-29612010000300012&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1984-29612010000300012&lng=en&nrm=iso
institution	REPOSITORIO UNB
collection	REPOSITORIO UNB
language	English
topic	Anaplasma marginale real time PCR cattle campeiro deer Pantanal Anaplasma marginale PCR tempo real bovino veado campeiro Pantanal
spellingShingle	Anaplasma marginale real time PCR cattle campeiro deer Pantanal Anaplasma marginale PCR tempo real bovino veado campeiro Pantanal Picoloto, Graziela Lima, Renileide Ferreira de Olegário, Lílian Andressa Oliveira Carvalho, Cristiano Miranda Espínola Lacerda, Ana Crystina Reis Tomás, Walfrido Moraes Borges, Paulo André Lima Pellegrin, Aiesca Oliveira Madruga, Cláudio Roberto Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
description	Epizootiological study of Anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. To achieve diagnosis of this rickettsia in cattle and campeiro deer of Brazilian Pantanal, a comparison was made between a real time polymerase chain reaction (RT-PCR) with intercalating Sybr Green fluorochrome and primers based on msp5 gene of A. marginale; a conventional PCR (C-PCR); and parasitological examination using thin blood smear stained with Giemsa-MayGrunwald. Both PCRs showed good performance in the diagnosis of A. marginale in cattle, and were superior to the parasitological exam. The RT-PCR detected seven positive campeiro deer (16.3%). This rate was significantly higher compared to C-PCR, which identified one animal as positive (2.3%), and also compared to parasitological diagnosis, which did not find any positive animals. The dissociation temperature average of positive reactions in cattle (81.72 ºC ± 0.20) was identical to dissociation temperature found in the cervids (81.72 ºC ± 0.12), suggesting that both animal species were infected with A. marginale. We concluded that RT-PCR can be used for A. marginale diagnosis and in epizootiological studies of cattle and cervids; in spite of the small number of campeiro deer samples, the results indicated that this wildlife species has importance in the Anaplasma epizootiology in the Brazilian Pantanal.
format	Artigo
author	Picoloto, Graziela Lima, Renileide Ferreira de Olegário, Lílian Andressa Oliveira Carvalho, Cristiano Miranda Espínola Lacerda, Ana Crystina Reis Tomás, Walfrido Moraes Borges, Paulo André Lima Pellegrin, Aiesca Oliveira Madruga, Cláudio Roberto
author_sort	Picoloto, Graziela
title	Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
title_short	Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
title_full	Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
title_fullStr	Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
title_full_unstemmed	Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal
title_sort	real time polymerase chain reaction to diagnose anaplasma marginale in cattle and deer (ozotoceros bezoarticus leucogaster) of the brazilian pantanal
publisher	Colégio Brasileiro de Parasitologia Veterinária
publishDate	2017
url	http://repositorio.unb.br/handle/10482/27920
_version_	1641988802391048192
score	13.657419

Real time polymerase chain reaction to diagnose Anaplasma marginale in cattle and deer (Ozotoceros bezoarticus leucogaster) of the Brazilian Pantanal

Similar Items